Evidence map›Paper›PMID 34599813›Full record

ArticleG3 (Bethesda, Md.)2021

Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase.

Daniel P Nickerson, Monique A Quinn, Joshua M Milnes

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in G3 (Bethesda, Md.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Daniel P NickersonDepartment of Biology, California State University, San Bernardino, San Bernardino, CA 92407, USA.ORCID 0000-0001-6698-4223
Monique A QuinnDepartment of Biology, California State University, San Bernardino, San Bernardino, CA 92407, USA.
Joshua M MilnesDepartment of Biochemistry, University of Washington School of Medicine, Seattle, WA 98195-3750, USA.
California State University, San Bernardino · USUniversity of Washington · US

Funding

Dynamics of Endomembrane Docking and FusionR01GM077349 · NIGMS · UNIVERSITY OF WASHINGTON · PI MERZ, ALEXEY JARRELL · 2006 to 2024
$6.1M
A transmembrane Rab GTPase accelerating protein targeted to peroxisomesSC2GM140979 · NIGMS · CALIFORNIA STATE UNIV SAN BERNARDINO · PI NICKERSON, DANIEL P. · 2021 to 2023
$437k
NIGMS NIH HHS R01 GM077349NIGMS NIH HHS SC2 GM140979
6 · The paper itself

Abstract

Plasmid shuttle vectors capable of replication in both Saccharomyces cerevisiae and Escherichia coli and optimized for controlled modification in vitro and in vivo are a key resource supporting yeast as a premier system for genetics research and synthetic biology. We have engineered a series of yeast shuttle vectors optimized for efficient insertion, removal, and substitution of plasmid yeast replication loci, allowing generation of a complete set of integrating, low copy and high copy plasmids via predictable operations as an alternative to traditional subcloning. We demonstrate the utility of this system through modification of replication loci via Cre recombinase, both in vitro and in vivo, and restriction endonuclease treatments.

Indexed as

Genetic VectorsSaccharomyces cerevisiaeCloning, MolecularIntegrasesPlasmidsCre recombinaseIntegrasesCre recombinasegenetic engineeringhomologous recombinationmolecular cloningplasmidreplicationshuttle vectoryeast

Identifiers

PMID34599813
PMCPMC8664424
OpenAlexW3203610653

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.