ArticleGenome biology and evolution2021
A Minimally Morphologically Destructive Approach for DNA Retrieval and Whole-Genome Shotgun Sequencing of Pinned Historic Dipteran Vector Species.
Article in Genome biology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
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- Breaking the cold chain: solutions for room temperature preservation of mosquitoes leading to high quality reference genomes.GigaScience · 2026Article
- The Evolution of Bees: Insights from 'Omic Studies.Genome biology and evolution · 2026Review
- Non-destructive DNA extraction for recovering mitochondrial genomes from museum grasshopper specimens.PloS one · 2026Article
- Must Epidemiologically Impactful Vector Control Interventions Disrupt Mosquito Population Structure? A Case Study of a Cluster-Randomised Controlled Trial.Evolutionary applications · 2025Article
- A targeted amplicon sequencing panel for cost-effective high-throughput genotyping ofbioRxiv : the preprint server for biology · 2025Article
- Genomics of Neotropical biodiversity indicators: Two butterfly radiations with rampant chromosomal rearrangements and hybridization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Geometric morphometrics analysis of forensically importantForensic sciences research · 2025Article
- Facilitating high throughput collections-based genomics: a comparison of DNA extraction and library building methods.Scientific reports · 2025Article
- GenomeDelta: detecting recent transposable element invasions without repeat library.Genome biology · 2024Article
- Minimally destructive hDNA extraction method for retrospective genetics of pinned historical Lepidoptera specimens.Scientific reports · 2024Article
- Pathogen prospecting of museums: Reconstructing malaria epidemiology.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Improved species assignments across the entireFrontiers in genetics · 2024Article
- Genomes from historical Drosophila melanogaster specimens illuminate adaptive and demographic changes across more than 200 years of evolution.PLoS biology · 2023Article
- A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections.Insects · 2023Article
- Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control.Pathogens (Basel, Switzerland) · 2023Review
- Developing the Protocol Infrastructure for DNA Sequencing Natural History Collections.Biodiversity data journal · 2023Article
- DrosoPhyla: Resources for Drosophilid Phylogeny and Systematics.Genome biology and evolution · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Museum collections contain enormous quantities of insect specimens collected over the past century, covering a period of increased and varied insecticide usage. These historic collections are therefore incredibly valuable as genomic snapshots of organisms before, during, and after exposure to novel selective pressures. However, these samples come with their own challenges compared with present-day collections, as they are fragile and retrievable DNA is low yield and fragmented. In this article, we tested several DNA extraction procedures across pinned historic Diptera specimens from four disease vector genera: Anopheles, Aedes, Culex, and Glossina. We identify an approach that minimizes morphological damage while maximizing DNA retrieval for Illumina library preparation and sequencing that can accommodate the fragmented and low yield nature of historic DNA. We identify several key points in retrieving sufficient DNA while keeping morphological damage to a minimum: an initial rehydration step, a short incubation without agitation in a modified low salt Proteinase K buffer (referred to as "lysis buffer C" throughout), and critical point drying of samples post-extraction to prevent tissue collapse caused by air drying. The suggested method presented here provides a solid foundation for exploring the genomes and morphology of historic Diptera collections.
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