ArticleScientific reports2021
Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Qualification of a quantitative stability indicating potency assay for mRNA-LNP vaccine candidates.Gates open research · 2026Article
- High-throughput single-virion DNA-PAINT reveals structural diversity, cooperativity, and flexibility during selective packaging in influenza.Nucleic acids research · 2025Article
- Therapeutic Modulation of the Microbiome in Oncology: Current Trends and Future Directions.Current pharmaceutical biotechnology · 2025Review
- Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content.Advanced healthcare materials · 2025Article
- Article
- Rapid and specific detection of single nanoparticles and viruses in microfluidic laminar flow via confocal fluorescence microscopy.bioRxiv : the preprint server for biology · 2024Article
- Article
- Single-Molecule Visualization of Bunyavirus Genome Segments Using Fluorescence In Situ Hybridization.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Paired Capture and FISH Detection of Individual Virions Enable Cell-Free Determination of Infectious Titers.ACS sensors · 2023Article
- High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization.PLoS pathogens · 2023Article
- Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection.Sensors (Basel, Switzerland) · 2023Review
- Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection.Pathogens (Basel, Switzerland) · 2022Review
- Illuminating RNA biology through imaging.Nature cell biology · 2022Review
- Virus morphology: Insights from super-resolution fluorescence microscopy.Biochimica et biophysica acta. Molecular basis of disease · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.
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Registered trials
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