Evidence map›Paper›PMID 34599106›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

Structural origins of

Ruth M Saecker, James Chen, Courtney E Chiu, Brandon Malone, Johanna Sotiris, Mark Ebrahim, Laura Y Yen, Edward T Eng, Seth A Darst

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Structures ofScience advances · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Mechanism of mammalian transcriptional repression by noncoding RNA.Nature structural & molecular biology · 2025
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ruth M SaeckerLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065; rsaecker@rockefeller.edu darst@rockefeller.edu.ORCID 0000-0001-6361-7428
James ChenLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.
Courtney E ChiuLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.
Brandon MaloneLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065.ORCID 0000-0001-7752-9824
Johanna SotirisThe Evelyn Gruss Lipper Cryo-Electron Microscopy Resource Center, The Rockefeller University, New York, NY 10065.
Mark EbrahimThe Evelyn Gruss Lipper Cryo-Electron Microscopy Resource Center, The Rockefeller University, New York, NY 10065.
Laura Y YenThe National Resource of Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027.
Edward T EngThe National Resource of Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027.ORCID 0000-0002-8014-7269
Seth A DarstLaboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065; rsaecker@rockefeller.edu darst@rockefeller.edu.ORCID 0000-0002-8241-3153

Funding

TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7M
Structure, function, and regulation of the bacterial transcription cycleR35GM118130 · NIGMS · ROCKEFELLER UNIVERSITY · PI DARST, SETH A. · 2016 to 2025
$8.9M
NIGMS NIH HHS P41 GM103310NIGMS NIH HHS R35 GM118130
6 · The paper itself

Abstract

The first step in gene expression in all organisms requires opening the DNA duplex to expose one strand for templated RNA synthesis. In

Indexed as

DNA, BacterialDNA-Directed RNA PolymerasesEscherichia coliEscherichia coli InfectionsPromoter Regions, GeneticTranscription, GeneticDNA, BacterialDNA-Directed RNA Polymerasescryo-EMopen complexpromoter DNARNA polymerasetranscription

Identifiers

PMID34599106
PMCPMC8501879

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.