Evidence map›Paper›PMID 34597677›Full record

ArticleExperimental eye research2021

Age-related changes of lens stiffness in wild-type and Cx46 knockout mice.

Wiktor Stopka, Tom Libby, Stephanie Lin, Eddie Wang, Chun-Hong Xia, Xiaohua Gong

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Connexin Mutations and Hereditary Diseases.International journal of molecular sciences · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Wiktor StopkaUC Berkeley - UCSF Graduate Program in Bioengineering, University of California, Berkeley, CA, USA.
Tom LibbyCenter for Biological Inspiration in Education and Research (CiBER), University of California, Berkeley, CA, USA.
Stephanie LinSchool of Optometry and Vision Science Program, University of California, Berkeley, CA, USA.
Eddie WangSchool of Optometry and Vision Science Program, University of California, Berkeley, CA, USA.
Chun-Hong XiaSchool of Optometry and Vision Science Program, University of California, Berkeley, CA, USA.
Xiaohua GongUC Berkeley - UCSF Graduate Program in Bioengineering, University of California, Berkeley, CA, USA; School of Optometry and Vision Science Program, University of California, Berkeley, CA, USA. Electronic address: xgong@berkeley.edu.

Funding

Cataracts, Connexin Mutants and Genetic ModifiersR01EY013849 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI Xiaohua Gong · 2002 to 2026
$8.2M
NEI NIH HHS R01 EY013849
6 · The paper itself

Abstract

We have investigated how connexin 46 (Cx46) regulates lens stiffness by studying different Cx46 knockout (Cx46KO) mice. A modified muscle lever system was used to determine the lens stiffness of wild-type (WT) and Cx46KO mice at the C57BL/6J (B6) and the 129SvJae (129) strain backgrounds according to total lens displacement at the point of maximum force when fresh lenses were compressed with a maximum of 2 mN of force. In comparison to B6-WT controls, young and old B6-Cx46KO lenses showed 23% and 28% reductions in lens displacement, respectively. Comparing to 129-WT controls, old 129-Cx46KO lenses showed 50% reduction in the lens displacement while young 129-Cx46KO lenses displayed similar displacement. Old B6-Cx46KO and old 129-Cx46KO lenses showed almost identical lens displacement, 128 μm versus 127 μm. Morphological data revealed unique changes of peripheral fiber cell shapes in young B6-WT lenses but not in young B6-Cx46KO, 129-WT and 129-Cx46KO lenses. This work reveals Cx46 deletion increases the lens stiffness in both young and old mice at B6 strain background but only in old mice at 129 strain background which contains intermediate filament CP49 gene deletion. Cx46 impairment increases old mouse lens stiffness and may contribute to the development of presbyopia.

Indexed as

AgingAnimalsCataractConnexinsDisease Models, AnimalElasticityGap JunctionsLens, CrystallineMiceMice, Inbred C57BLMice, KnockoutConnexinsGJA3 protein, humanAgingCataractConnexinGap junctionIntermediate filamentLens

Identifiers

PMID34597677
PMCPMC10054276

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.