Evidence map›Paper›PMID 34596853›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

MOrPH-PhD: A Phage Display System for the Functional Selection of Genetically Encoded Macrocyclic Peptides.

Yu Gu, Jacob A Iannuzzelli, Rudi Fasan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yu GuDepartment of Chemistry, University of Rochester, Rochester, NY, USA.
Jacob A IannuzzelliDepartment of Chemistry, University of Rochester, Rochester, NY, USA.
Rudi FasanDepartment of Chemistry, University of Rochester, Rochester, NY, USA. rfasan@ur.rochester.edu.

Funding

Macrocyclic Peptide Modulators of Protein FunctionR01GM134076 · NIGMS · UNIVERSITY OF TEXAS DALLAS · PI FASAN, RUDI · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM134076
6 · The paper itself

Abstract

Macrocyclic peptides represent promising scaffolds for targeting biomolecules with high affinity and selectivity, making methods for the diversification and functional selection of these macrocycles highly valuable for drug discovery purposes. We recently reported a novel phage display platform (called MOrPH-PhD) for the creation and functional exploration of combinatorial libraries of genetically encoded cyclic peptides. In this system, spontaneous, posttranslational peptide cyclization by means of a cysteine-reactive non-canonical amino acid is integrated with M13 bacteriophage display, enabling the creation of genetically encoded macrocyclic peptide libraries displayed on phage particles. Using this system, it is possible to rapidly generate and screen large libraries of phage-displayed macrocyclic peptides (up to 10

Indexed as

Peptide LibraryBacteriophagesCell Surface Display TechniquesPeptidesPeptides, CyclicPeptide LibraryPeptidesPeptides, CyclicDrug discoveryMacrocyclic peptidesNoncanonical amino acidsPhage displayProtein–protein interactions

Identifiers

PMID34596853
PMCPMC8493807

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.