Evidence map›Paper›PMID 34596849›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Protein Catalyzed Capture (PCC) Agents for Antigen Targeting.

Matthew N Idso, Bert T Lai, Heather D Agnew, James R Heath

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Matthew N IdsoInstitute for Systems Biology, Seattle, WA, USA.
Bert T LaiIndi Molecular, Inc., Culver City, CA, USA.
Heather D AgnewIndi Molecular, Inc., Culver City, CA, USA.
James R HeathInstitute for Systems Biology, Seattle, WA, USA. jim.heath@isbscience.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The protein catalyzed capture agent (PCC) method is a powerful combinatorial screening strategy for discovering synthetic macrocyclic peptide ligands, called PCCs, to designated protein epitopes. The foundational concept of the PCC method is the use of in situ click chemistry to survey large combinatorial libraries of peptides for ligands to designated biological targets. State-of-the-art PCC screens integrate synthetic libraries of constrained macrocyclic peptides with epitope-specific targeting strategies to identify high-affinity (<100 nM) binders de novo. Automated instrumentation can accelerate PCC discovery to a rapid 2-week timeframe. Here, we describe methods to perform combinatorial screens that yield epitope-targeted PCCs.

Indexed as

Peptide LibraryCatalysisCombinatorial Chemistry TechniquesEpitopesLigandsPeptidesProteinsEpitopesLigandsPeptide LibraryPeptidesProteinsCombinatorial chemistryEpitope targetedIn situ click chemistryMacrocyclic peptidesOne-bead one-compound libraries

Identifiers

PMID34596849

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.