ArticleChina CDC weekly2020
Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus.
Article in China CDC weekly, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- Trial
- Wastewater-Based Surveillance of SARS-CoV-2 for Early Warning of COVID-19 Infection Dynamics.Viruses · 2026Article
- Wastewater-based surveillance as a proactive public health tool: insights from SARS-CoV-2 monitoring in Kampala, Uganda (2023-2024).BMC public health · 2026Article
- Post-Mortem Community Surveillance of COVID-19: Implementation and Evaluation of a Pilot System in the Funeral Sector in England, UK, January 2021 to February 2022.Influenza and other respiratory viruses · 2025Article
- Using machine learning models to predict the impact of template mismatches on polymerase chain reaction assay performance.Scientific reports · 2025Article
- Comprehensive Analysis of SARS-CoV-2 Dynamics in Bangladesh: Infection Trends and Variants (2020-2023).Viruses · 2024Article
- Detecting SARS-CoV-2 BA.2, BA.4, and BA.5 Variants Utilizing a Robust RT-RPA-CRISPR/Cas12a-Based Method - China, 2023.China CDC weekly · 2023Article
- A review of current effective COVID-19 testing methods and quality control.Archives of microbiology · 2023Review
- Evaluation of real-time NASBA assay for the detection of SARS-CoV-2 compared with real-time PCR.Irish journal of medical science · 2023Article
- Rapid inactivation and sample preparation for SARS-CoV-2 PCR-based diagnostics using TNA-Cifer Reagent E.Frontiers in microbiology · 2023Article
- Review
- Identification of mutations in SARS-CoV-2 PCR primer regions.Scientific reports · 2022Article
- Development of an efficient wastewater testing protocol for high-throughput country-wide SARS-CoV-2 monitoring.The Science of the total environment · 2022Article
- Diagnostic assay and technology advancement for detecting SARS-CoV-2 infections causing the COVID-19 pandemic.Analytical and bioanalytical chemistry · 2022Review
- Emergence of the delta variant and risk of SARS-CoV-2 infection in secondary school students and staff: Prospective surveillance in 18 schools, England.EClinicalMedicine · 2022Article
- SARS-CoV-2 detection in nasopharyngeal swabs: Performance characteristics of a real-time RT-qPCR and a droplet digital RT-PCR assay based on the exonuclease region (ORF1b, nsp 14).Journal of virological methods · 2022Article
- Factors influencing the performance of rapid SARS-CoV-2 antigen tests under field condition.Journal of clinical laboratory analysis · 2022Article
- Impact of SARS-CoV-2 Mutations on PCR Assay Sequence Alignment.Frontiers in public health · 2022Article
- Considerations about Reducing False-Negative PCR Test for COVID-19.International journal of MCH and AIDS · 2022Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
WHAT IS ALREADY KNOWN ON THIS TOPIC?: A novel human coronavirus, known as SARS-CoV-2 or 2019-nCoV, is the causative agent of the coronavirus disease 2019 (COVID-19). We have released the primers and probes of real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the laboratory detection of COVID-19 infection. WHAT IS ADDED BY THIS REPORT?: Here we provide detailed technical data and evaluate the performance of three novel rRT-PCR assays targeting the ORF1ab, N, and E genes for detection of COVID-19 infection. The application of rRT-PCR assays among four types of specimens (alveolar lavage, sputum, throat swabs, and stool) from patients with COVID-19 indicated that the mean viral loads detected in sputum were higher than other specimens. WHAT ARE THE IMPLICATIONS FOR PUBLIC HEALTH PRACTICE?: These rRT-PCR assays reported here could be used for laboratory diagnosis of COVID-19 infection with high sensitivity, specificity, and applicability. Sputum rather than throat swabs and stool should be a priority for specimen collection for laboratory detection of COVID-19.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.