ArticleEcology and evolution2021
Optimizing noninvasive sampling of a zoonotic bat virus.
Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- Paramyxoviruses in Old World fruit bats (Pteropodidae): An open database and synthesis of sampling effort, viral positivity, and coevolution.PLoS neglected tropical diseases · 2025Pooled it
- Coronavirus sampling and surveillance in bats from 1996-2019: a systematic review and meta-analysis.Nature microbiology · 2023Pooled it
- Periodic shifts in viral load increase risk of Hendra virus spillover fromScience advances · 2026Article
- Review
- A paired analysis of mercury among non-invasive tissues in Mexican free-tailed bats (Tadarida brasiliensis) to inform conservation monitoring.Ecotoxicology (London, England) · 2025Article
- Synchronized seasonal excretion of multiple coronaviruses coincides with high rates of coinfection in immature bats.Nature communications · 2025Article
- A field guide for sampling bats (Chiroptera) for eco-epidemiological studies.Frontiers in veterinary science · 2025Review
- A paired analysis of mercury among non-invasive tissues to inform bat conservation monitoring.bioRxiv : the preprint server for biology · 2024Article
- The latest advancements in Sosuga virus (SOSV) research.Frontiers in microbiology · 2024Review
- Metagenomic and Molecular Detection of Novel Fecal Viruses in Free-Ranging Agile Wallabies.EcoHealth · 2023Article
- Trends in Bacterial Pathogens of Bats: Global Distribution and Knowledge Gaps.Transboundary and emerging diseases · 2023Review
- Nipah Virus Detection at Bat Roosts after Spillover Events, Bangladesh, 2012-2019.Emerging infectious diseases · 2022Article
- Seasonality of coronavirus shedding in tropical bats.Royal Society open science · 2022Article
- Optimizing noninvasive sampling of a zoonotic bat virus.Ecology and evolution · 2021Article
- From Protein to Pandemic: The Transdisciplinary Approach Needed to Prevent Spillover and the Next Pandemic.Viruses · 2021Review
- Sampling to elucidate the dynamics of infections in reservoir hosts.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Outbreaks of infectious viruses resulting from spillover events from bats have brought much attention to bat-borne zoonoses, which has motivated increased ecological and epidemiological studies on bat populations. Field sampling methods often collect pooled samples of bat excreta from plastic sheets placed under-roosts. However, positive bias is introduced because multiple individuals may contribute to pooled samples, making studies of viral dynamics difficult. Here, we explore the general issue of bias in spatial sample pooling using Hendra virus in Australian bats as a case study. We assessed the accuracy of different under-roost sampling designs using generalized additive models and field data from individually captured bats and pooled urine samples. We then used theoretical simulation models of bat density and under-roost sampling to understand the mechanistic drivers of bias. The most commonly used sampling design estimated viral prevalence 3.2 times higher than individual-level data, with positive bias 5-7 times higher than other designs due to spatial autocorrelation among sampling sheets and clustering of bats in roosts. Simulation results indicate using a stratified random design to collect 30-40 pooled urine samples from 80 to 100 sheets, each with an area of 0.75-1 m
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.