Evidence map›Paper›PMID 34594460›Full record

ArticleJournal of microbiology & biology education2021

CRISPR-Cas9 Gene Editing in Yeast: A Molecular Biology and Bioinformatics Laboratory Module for Undergraduate and High School Students.

Saumya M Sankaran, Justin D Smith, Kevin R Roy

Open access · goldAbstract read
In one paragraph

Article in Journal of microbiology & biology education, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Using CRISPRi inJournal of microbiology & biology education · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Saumya M SankaranDepartment of Biomedical Science, Kaiser Permanente Bernard J. Tyson School of Medicine, Pasadena, California, USA.
Justin D SmithStanford Genome Technology Center, Stanford University, Palo Alto, California, USA.
Kevin R RoyStanford Genome Technology Center, Stanford University, Palo Alto, California, USA.
Stanford University · USKaiser Permanente · US

Funding

Capturing the phenotypic landscape of single-nucleotide variation via systematic genome editingR01GM121932 · NIGMS · STANFORD UNIVERSITY · PI STEINMETZ, LARS M · 2017 to 2021
$3.4M
NIGMS NIH HHS R01 GM121932
6 · The paper itself

Abstract

CRISPR-Cas9 genome editing technology is widely used in scientific research and biotechnology. As this technology becomes a staple tool in life sciences research, it is increasingly important to incorporate it into biology curricula to train future scientists. To demonstrate the molecular underpinnings and some limitations of CRISPR-based gene editing, we designed a laboratory module to accompany a discussion-based course on genome editing for college and advanced high school biology students. The laboratory module uses CRISPR-Cas9 to target and inactivate the

Indexed as

college biology labCRISPRCRISPR-Cas9DNA repairgene editinghigh school biology lablaboratory moduleproject-based labyeast

Identifiers

PMID34594460
PMCPMC8442027
OpenAlexW3170442369

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.