ArticleNature methods2021
High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy.
Article in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 1 of them a synthesis that pooled it.
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Who cites it
77 citing papers in PubMed, 1 synthesis or guideline pooled it, 166 citations in OpenAlex.
- Neuroimaging Insights into the Public Health Burden of Neuropsychiatric Disorders: A Systematic Review of Electroencephalography-Based Cognitive Biomarkers.Medicina (Kaunas, Lithuania) · 2025Pooled it
- Rapid robust high-fidelity 3D neuronal extraction from multiview calcium imaging datasets.Nature methods · 2026Article
- Depth-adapted adaptive optics for three-photon microscopy.Biomedical optics express · 2026Article
- Graph-based modeling of optical system enables adaptive optics on dynamic samples with self-calibration.iScience · 2026Article
- Inverse scattering in biological samples via beam propagation.Science advances · 2026Article
- Physics-informed multi-encoder adaptive optics enables rapid aberration correction for intravital microscopy of deep complex tissue.Nature communications · 2026Article
- Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields.Nature methods · 2026Article
- Self-localized ultrafast pencil beam for volumetric multiphoton imaging.Nature methods · 2026Article
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- High-fidelity intravital imaging of biological dynamics with latent-space-enhanced digital adaptive optics.Nature biotechnology · 2026Article
- Long-wavelength oblique back-illumination microscopy for deepBiomedical optics express · 2026Article
- Three-photon imaging of hippocampal neurogenesis through the intact mouse brain.Research square · 2025Article
- Review of shortwave infrared imaging and spectroscopy in tissue [Invited].Biomedical optics express · 2025Review
- Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice.Nature communications · 2025Article
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- Formulation Strategies for Immunomodulatory Natural Products in 3D Tumor Spheroids and Organoids: Current Challenges and Emerging Solutions.Pharmaceutics · 2025Review
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Compressive Fourier-Domain Intensity Coupling (C-FOCUS) enables near-millimeter deep imaging in the intact mouse brain in vivo.Research square · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Technology Roadmap of Micro/Nanorobots.ACS nano · 2025Review
17 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiphoton microscopy has become a powerful tool with which to visualize the morphology and function of neural cells and circuits in the intact mammalian brain. However, tissue scattering, optical aberrations and motion artifacts degrade the imaging performance at depth. Here we describe a minimally invasive intravital imaging methodology based on three-photon excitation, indirect adaptive optics (AO) and active electrocardiogram gating to advance deep-tissue imaging. Our modal-based, sensorless AO approach is robust to low signal-to-noise ratios as commonly encountered in deep scattering tissues such as the mouse brain, and permits AO correction over large axial fields of view. We demonstrate near-diffraction-limited imaging of deep cortical spines and (sub)cortical dendrites up to a depth of 1.4 mm (the edge of the mouse CA1 hippocampus). In addition, we show applications to deep-layer calcium imaging of astrocytes, including fibrous astrocytes that reside in the highly scattering corpus callosum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.