ArticleNature methods2021
Image-guided MALDI mass spectrometry for high-throughput single-organelle characterization.
Article in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 88 citations in OpenAlex.
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- Protocol for reconstructing 2D and 3D peptide atlases of the Ciona cerebral ganglion at single-cell resolution.STAR protocols · 2026Article
- SPOTTER: Automated Tissue-Barcoding Platform for Spatial Proteomics and Phosphoproteomics.bioRxiv : the preprint server for biology · 2026Article
- Small is big: growing impact of small molecule mass spectrometry in infectious disease drug development.mSphere · 2026Review
- Proteoform profiling of endogenous single cells from rat hippocampus at scale.Nature biotechnology · 2026Article
- Single-cell thiol profiling enabled by live-cell labeling reveals metabolic heterogeneity in ferroptosis.Nature communications · 2026Article
- Interface-engineered plasmonic covalent organic framework nanofilms on TiOScience advances · 2026Article
- Multimodal mass spectrometry imaging for plaque- and region-specific neurolipidomics in Alzheimer's disease mouse models.Nature communications · 2025Article
- Direct analysis of biotransformations with mass spectrometry-DiBT-MS.Nature protocols · 2025Review
- Two- and three-dimensional neuropeptidomic landscape in the central nervous system of an invertebrate chordate,iScience · 2025Article
- Technologies for Decoding Cancer Metabolism with Spatial Resolution.Cold Spring Harbor perspectives in medicine · 2025Review
- Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells.Journal of visualized experiments : JoVE · 2025Article
- A Primer on Proteomic Characterization of Intercellular Communication in a Virus Microenvironment.Molecular & cellular proteomics : MCP · 2025Review
- Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping.The Plant cell · 2025Article
- Mass spectrometry imaging for unearthing and validating quality markers in traditional Chinese medicines.Chinese herbal medicines · 2025Review
- Lipidomics and temporal-spatial distribution of organelle lipid.Journal of biological methods · 2025Review
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- MALDI MSI Protocol for Spatial Bottom-Up Proteomics at Single-Cell Resolution.Journal of proteome research · 2024Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
Peptidergic dense-core vesicles are involved in packaging and releasing neuropeptides and peptide hormones-critical processes underlying brain, endocrine and exocrine function. Yet, the heterogeneity within these organelles, even for morphologically defined vesicle types, is not well characterized because of their small volumes. We present image-guided, high-throughput mass spectrometry-based protocols to chemically profile large populations of both dense-core vesicles and lucent vesicles for their lipid and peptide contents, allowing observation of the chemical heterogeneity within and between these two vesicle populations. The proteolytic processing products of four prohormones are observed within the dense-core vesicles, and the mass spectral features corresponding to the specific peptide products suggest three distinct dense-core vesicle populations. Notable differences in the lipid mass range are observed between the dense-core and lucent vesicles. These single-organelle mass spectrometry approaches are adaptable to characterize a range of subcellular structures.
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