ArticleHorticulture research2021
Development of potent promoters that drive the efficient expression of genes in apple protoplasts.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- A dual-platform toolkit for transient gene expression and genome editing in rubber dandelion (aBIOTECH · 2026Article
- An efficient method for tissue-specific protoplast isolation suitable for single-cell RNA sequencing and transient gene expression analysis in saffron (Crocus sativus L.).BMC biotechnology · 2026Article
- Protoplast-Based Functional Genomics and Genome Editing: Progress, Challenges and Applications.Plant, cell & environment · 2026Review
- Root-Specific Signal Modules Mediating Abiotic Stress Tolerance in Fruit Crops.Plants (Basel, Switzerland) · 2026Review
- The peanutaBIOTECH · 2025Article
- Rvi6-mediated defense mechanisms against apple scab.BMC plant biology · 2025Article
- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- Viral-Based Gene Editing System for Nutritional Improvement of Fructan Content in Lettuce.International journal of molecular sciences · 2025Article
- A Comparison of DNA-Methylation during Protoplast Culture of Ponkan Mandarin (Plants (Basel, Switzerland) · 2024Article
- Identification of osmotic stress resistance mediated byFrontiers in plant science · 2024Article
- Application of a novel strong promoter from Chinese fir (Frontiers in plant science · 2023Article
- Protoplast isolation and transient transformation system forFrontiers in plant science · 2023Article
- Article
- Advances in protoplast transfection promote efficient CRISPR/Cas9-mediated genome editing in tetraploid potato.Planta · 2022Article
- Heterologous Biosynthesis of Health-Promoting Baicalein inMolecules (Basel, Switzerland) · 2022Article
- Design and Analysis of Native Photorespiration Gene Motifs of Promoter Untranslated Region Combinations Under Short Term Abiotic Stress Conditions.Frontiers in plant science · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Protoplast transient expression is a powerful strategy for gene functional characterization, especially in biochemical mechanism studies. We herein developed a highly efficient transient expression system for apple protoplasts. The abilities of the Arabidopsis thaliana and Malus domestica ubiquitin-10 (AtUBQ10 and MdUBQ10) promoters to drive the expression of multiple genes were compared with that of the CaMV 35S promoter, and the results revealed that the AtUBQ10 and MdUBQ10 promoters were more efficient in apple protoplasts. With this system, we demonstrated that active AtMKK7ac could activate MAPK6/3/4 signaling cascades, which further regulated MdWRKY33 phosphorylation and stability in apple. Furthermore, the ligand-induced interaction between the immune receptor AtFLS2 and the coreceptor AtBAK1 was reconstituted in apple protoplasts. We also found that the stability of the bacterial effector AvrRpt2 was regulated by feedback involving auxin and the immune regulator RIN4. The system established herein will serve as a useful tool for the molecular and biochemical analyses of apple genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.