ArticleNucleic acids research2021
A pathogen-specific sRNA influences enterohemorrhagic Escherichia coli fitness and virulence in part by direct interaction with the transcript encoding the ethanolamine utilization regulatory factor EutR.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 17 citations in OpenAlex.
- Small Regulatory RNAs in Prokaryotes: Key Features, Identification, Environmental Roles, and Applications.Microorganisms · 2026Review
- EnterohemorrhagicmSystems · 2026Article
- Global Proteomic Analysis Reveals the Roles of MicX in Biofilm Formation and Quorum Sensing inFoods (Basel, Switzerland) · 2026Article
- sRNA centered signaling activates nitrate respiration and enhances Cronobacter sakazakii virulence in host environments.Nature communications · 2026Article
- A small RNA fromVirulence · 2025Article
- Role of Small Non-Coding RNA in Gram-Negative Bacteria: New Insights and Comprehensive Review of Mechanisms, Functions, and Potential Applications.Molecular biotechnology · 2025Review
- The type 6 secretion system immunity protein RhsFI has been repurposed for small RNA regulation in pathogenic Escherichia coli.Nucleic acids research · 2025Article
- Low leucine levels in the blood enhance the pathogenicity of neonatal meningitis-causing Escherichia coli.Nature communications · 2025Article
- The RNA-binding protein ProQ directly binds and regulates virulence genes in enterohemorrhagicFrontiers in cellular and infection microbiology · 2025Article
- ThemSphere · 2024Article
- A novel small RNA regulates Locus of Enterocyte Effacement and site-specific colonization of enterohemorrhagicFrontiers in cellular and infection microbiology · 2024Article
- Genome-wide sRNA and mRNA transcriptomic profiling insights into carbapenem-resistantFrontiers in cellular and infection microbiology · 2024Article
- Pathogenicity and identification of host adaptation genes of the avian pathogenicFrontiers in cellular and infection microbiology · 2024Article
- The two sRNAs OmrA and OmrB indirectly repress transcription from the LEE1 promoter of enteropathogenic Escherichia coli.Folia microbiologica · 2023Article
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2 authors at 1 institution in 1 country.
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Abstract
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 relies on sRNAs to coordinate expression of metabolic and virulence factors to colonize the host. Here, we focus on the sRNA, named MavR (metabolism and virulence regulator), that is conserved among pathogenic Enterobacteriaceae. MavR is constitutively expressed under in vitro conditions that promote EHEC virulence gene expression. Using MS2-affinity purification coupled with RNA sequencing, the eutR transcript was identified as a putative target of MavR. EutR is a transcription factor that promotes expression of genes required for ethanolamine metabolism as well as virulence factors important for host colonization. MavR binds to the eutR coding sequence to protect the eutR transcript from RNase E-mediated degradation. Ultimately, MavR promotes EutR expression and in turn ethanolamine utilization and ethanolamine-dependent growth. RNAseq analyses revealed that MavR also affected expression of genes important for other metabolic pathways, motility, oxidative stress and attaching and effacing lesion formation, which contribute to EHEC colonization of the gastrointestinal tract. In support of the idea that MavR-dependent gene expression affects fitness during infection, deletion of mavR resulted in significant (∼10- to 100-fold) attenuation in colonization of the mammalian intestine. Altogether, these studies reveal an important, extensive, and robust phenotype for a bacterial sRNA in host-pathogen interactions.
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