Evidence map›Paper›PMID 34591312›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Subcellular Force Quantification of Endothelial Cells Using Silicone Pillar Arrays.

Mohanish K Chandurkar, Sangyoon J Han

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Mohanish K ChandurkarDepartment of Biomedical Engineering, Michigan Technological University, Houghton, MI, USA.
Sangyoon J HanDepartment of Biomedical Engineering, Michigan Technological University, Houghton, MI, USA. sjhan@mtu.edu.

Funding

Nascent Adhesion-Based Mechano-transmission for Extracellular Matrix Stiffness SensingR15GM135806 · NIGMS · MICHIGAN TECHNOLOGICAL UNIVERSITY · PI HAN, SANGYOON JOSHUA · 2019 to 2024
$964k
NIGMS NIH HHS R15 GM135806
6 · The paper itself

Abstract

Micropillar arrays are one of the tools that quantify the mechanical forces exerted by endothelial cells and any other adherent cell types. This chapter describes the fabrication and usage of the micropillars that reports spatial distribution of traction forces by endothelial cells. The fabrication begins with making a "master" pillar substrate using photolithography, followed by softlithography of the master with polydimethylsiloxane. The pillars can then be printed with an extracellular matrix protein in specific shape using stamp-off technique. Lastly, imaging of the micropillars with cells and the analysis of the traction force using Matlab-based software are described.

Indexed as

Endothelial CellsCell AdhesionMechanical PhenomenaSiliconesSiliconesEndothelial cellsExtracellular matrixFocal adhesionMicropillarSoftlithographyTraction force microscopy

Identifiers

PMID34591312
PMCPMC11139549

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.