Evidence map›Paper›PMID 34591125›Full record

ArticleExperimental brain research2021

Kcnk3, Ggta1, and Gpr84 are involved in hyperbaric oxygenation preconditioning protection on cerebral ischemia-reperfusion injury.

Chunhui Yang, Minjun Ding, Guiqiang Shao, Shengjie Jia, Xue Yin, Yuhui Cui, Zetian Wang, Chunrong Wu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental brain research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Illuminating proinflammatory myeloid cells with PET tracers targeting GPR84.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Chunhui Yang *Department of Trauma-Emergency and Critical Care Medicine Center (TECCMC), The Fifth People Hospital of Shanghai Fudan University, Shanghai, 200240, China.
Minjun Ding *Department of Trauma-Emergency and Critical Care Medicine Center (TECCMC), The Fifth People Hospital of Shanghai Fudan University, Shanghai, 200240, China.
Guiqiang ShaoDepartment of Hyperbaric Oxygen Chamber, Wujing Hospital, Minhang District, Shanghai, 200241, China.
Shengjie JiaDepartment of Trauma-Emergency and Critical Care Medicine Center (TECCMC), The Fifth People Hospital of Shanghai Fudan University, Shanghai, 200240, China.
Xue YinDepartment of Trauma-Emergency and Critical Care Medicine Center (TECCMC), The Fifth People Hospital of Shanghai Fudan University, Shanghai, 200240, China.
Yuhui CuiDepartment of Trauma-Emergency and Critical Care Medicine Center (TECCMC), The Fifth People Hospital of Shanghai Fudan University, Shanghai, 200240, China.
Zetian WangDepartment of Trauma-Emergency and Critical Care Medicine Center (TECCMC), The Fifth People Hospital of Shanghai Fudan University, Shanghai, 200240, China.
Chunrong WuDepartment of Trauma-Emergency and Critical Care Medicine Center (TECCMC), The Fifth People Hospital of Shanghai Fudan University, Shanghai, 200240, China. bamboo_1985@126.com.
Fudan University · CNMinhang District Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study aimed to explore the potential mechanism of the effect of hyperbaric oxygenation (HBO) preconditioning on cerebral ischemia and reperfusion injury (CIRI). GSE23160 dataset was used to identify differentially expressed genes (DEGs) from striatum between the middle cerebral artery occlusion (MCAO)/reperfusion and sham rats. The gene clusters with continuous increase and decrease were identified by soft clustering analysis in Mfuzz, and functional enrichment analysis of these genes was performed using clusterProfiler package. The intersection set of the genes with significantly altered expression at post-reperfusion 2, 8, and 24 h were screened in comparison to 0 h (sham group), and the expression of these genes was detected in the MCAO/reperfusion model and HBO preconditioning groups by real-time PCR (RT-PCR) and western blotting. A total of 41 upregulated DEGs, and 7 downregulated DEGs were detected, among which the expression of Gpr84 and Ggta1 was significantly upregulated at each reperfusion phase as compared to the sham group, while the expression of Kcnk3 was significantly downregulated except in the postreperfusion 8 h in the striatum group. RT-PCR and western blotting analyses showed that the expression of Ggta1, Gpr84, and Kcnk3 genes between the MCAO/reperfusion and sham rats were consistent with the bioinformatics analysis. In addition, the HBO preconditioning reduced the expression of Ggta1 and Gpr84 and increased the expression of Kcnk3 in MCAO/reperfusion rats. Kcnk3, Ggta1, and Gpr84 may play a major role in HBO-mediated protection of the brain against CIRI.

Indexed as

Brain IschemiaHyperbaric OxygenationReperfusion InjuryAnimalsInfarction, Middle Cerebral ArteryRatsRats, Sprague-DawleyCerebral ischemia and reperfusion injuryGgta1Gpr84Hyperbaric oxygenation preconditioningKcnk3Middle cerebral artery occlusion/reperfusion

Identifiers

PMID34591125
PMCPMC8599220
OpenAlexW3204529875

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.