Evidence map›Paper›PMID 34590290›Full record

ArticleHuman cell2022

Establishment of Down's syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT.

Takeyoshi Asakawa, Atsushi Yamada, Masumi Kugino, Tomokazu Hasegawa, Kentaro Yoshimura, Kiyohito Sasa, Mitsuhiro Kinoshita, Masakazu Nitta, Karin Nagata, Tomomi Sugiyama and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Human cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Establishment of an immortalized yak granulosa cell line:Frontiers in veterinary science · 2024
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Takeyoshi AsakawaDepartments of Pediatric Dentistry, School of Dentistry, Showa University, Tokyo, Japan. tasakawa@dent.showa-u.ac.jp.ORCID http://orcid.org/0000-0002-7953-5600
Atsushi YamadaDepartments of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Masumi KuginoDepartments of Pediatric Dentistry, School of Dentistry, Showa University, Tokyo, Japan.
Tomokazu HasegawaDepartment of Pediatric Dentistry, Institute of Health Bioscience, Tokushima University, Tokushima, Japan.
Kentaro YoshimuraDepartments of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Kiyohito SasaDepartments of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Mitsuhiro KinoshitaDepartments of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Masakazu NittaDepartments of Pediatric Dentistry, School of Dentistry, Showa University, Tokyo, Japan.
Karin NagataDepartments of Pediatric Dentistry, School of Dentistry, Showa University, Tokyo, Japan.
Tomomi SugiyamaDepartments of Pediatric Dentistry, School of Dentistry, Showa University, Tokyo, Japan.
Ryutaro KamijoDepartments of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Takahiro FunatsuDepartments of Pediatric Dentistry, School of Dentistry, Showa University, Tokyo, Japan.
Showa University · JPTokushima University · JP

Funding

Japan Society for the Promotion of Science 16K11812Japan Society for the Promotion of Science 19H03820
6 · The paper itself

Abstract

Down's syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down's syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down's syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down's syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of DSCR-1 in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down's syndrome patients.

Indexed as

Down SyndromeAntigens, Polyomavirus TransformingCell LineDNA-Binding ProteinsGene ExpressionHealthy VolunteersHumansMaleMiddle AgedMuscle ProteinsPeptide FragmentsPeriodontal DiseasesPeriodontal LigamentTelomeraseTransfectionAntigens, Polyomavirus TransformingDNA-Binding ProteinsGV1001 peptideMuscle ProteinsPeptide FragmentsRCAN1 protein, humanTelomeraseDown’s syndromeDSCR-1hTERTPeriodontal ligament cellsSV40T-Ag

Identifiers

PMID34590290
PMCPMC8732922
OpenAlexW3204538293

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.