Evidence map›Paper›PMID 34590261›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Monitoring Persister Resuscitation with Flow Cytometry.

Sayed Golam Mohiuddin, Mehmet A Orman

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Sayed Golam MohiuddinDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, USA.
Mehmet A OrmanDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, USA. morman@central.uh.edu.

Funding

Characterizing a Self-Digesting-Mediated Reversible Drug Tolerance Mechanism in BacteriaR01AI143643 · NIAID · UNIVERSITY OF HOUSTON · PI ORMAN, MEHMET A. · 2020 to 2024
$1.9M
Investigating "viable but non-culturable" Cell and Persister Formation MechanismsK22AI125468 · NIAID · UNIVERSITY OF HOUSTON · PI ORMAN, MEHMET A. · 2018 to 2019
$270k
NIAID NIH HHS K22 AI125468NIAID NIH HHS R01 AI143643
6 · The paper itself

Abstract

Persister cells are defined as a small fraction of phenotypic variants in a cell population that are temporarily tolerant to bactericidal antibiotics. Persisters are not mutant cells; they generally survive lethal concentrations of antibiotics due to their transient nongrowing state. Persister cells have the ability to resuscitate after the end of antibiotic treatment. Despite significant advancements in the understanding of the molecular mechanisms underlying persister formation, we still have little information about their resuscitation mechanisms. In this chapter, we describe a method to detect and monitor persister resuscitation at the single-cell level using flow cytometry analysis. This method enables us to not only assess the resuscitation characteristics of persisters but also determine and quantify various subpopulations in antibiotic-treated cultures, including viable but nonculturable (VBNC) and dead cells.

Indexed as

Flow CytometryAnti-Bacterial AgentsEscherichia coliMicrobial ViabilityAnti-Bacterial AgentsAntibiotic toleranceBeta lactamsEscherichia coliFlow cytometryPersister resuscitationProtein dilutionViable but nonculturable cells

Identifiers

PMID34590261

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.