ArticleScientific reports2021
Molecular principles of recruitment and dynamics of guest proteins in liquid droplets.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
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- Nanoscale domains govern local diffusion and ageing within fused-in-sarcoma condensates.Nature nanotechnology · 2026Article
- Measuring concentration and diffusivity within biomolecular condensates using calibration-free scanning fluorescence correlation spectroscopy.Chemical science · 2026Article
- Nanocluster intermediates orchestrate the phase transition of ATP condensates.Communications chemistry · 2025Article
- Evolutionarily conserved grammar rules viral factories of amoeba-infecting members of the hyperdiverseProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid-induced condensate formation from the Alzheimer's Aβ peptide triggers amyloid aggregation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CLASP-mediated competitive binding in protein condensates directs microtubule growth.Nature communications · 2024Article
- Phosphomimetic substitutions in TDP-43's transiently α-helical region suppress phase separation.Biophysical journal · 2024Article
- Cross-Talk of Cation-π Interactions with Electrostatic and Aromatic Interactions: A Salt-Dependent Trade-off in Biomolecular Condensates.The journal of physical chemistry letters · 2023Article
- Single-molecule characterization of target search dynamics of DNA-binding proteins in DNA-condensed droplets.Nucleic acids research · 2023Article
- Rational design of phase separating peptides based on phase separating protein sequence of p53.Scientific reports · 2023Article
- Rational peptide design for regulating liquid-liquid phase separation on the basis of residue-residue contact energy.Scientific reports · 2022Article
- Structure-dependent recruitment and diffusion of guest proteins in liquid droplets of FUS.Scientific reports · 2022Article
- Protein conformation and biomolecular condensates.Current research in structural biology · 2022Review
- Single-Molecule Microscopy Meets Molecular Dynamics Simulations for Characterizing the Molecular Action of Proteins on DNA and in Liquid Condensates.Frontiers in molecular biosciences · 2021Review
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Authors and funding
14 authors at 5 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Despite the continuous discovery of host and guest proteins in membraneless organelles, complex host-guest interactions hinder the understanding of the molecular grammar governing liquid-liquid phase separation. In this study, we characterized the localization and dynamic properties of guest proteins in liquid droplets using single-molecule fluorescence microscopy. Eighteen guest proteins of different sizes, structures, and oligomeric states were examined in host p53 liquid droplets. Recruitment did not significantly depend on the structural properties of the guest proteins, but was moderately correlated with their length, total charge, and number of R and Y residues. In contrast, the diffusion of disordered guest proteins was comparable to that of host p53, whereas that of folded proteins varied widely. Molecular dynamics simulations suggest that folded proteins diffuse within the voids of the liquid droplet while interacting weakly with neighboring host proteins, whereas disordered proteins adapt their structures to form tight interactions with the host proteins. Our study provides insights into the key molecular principles of the localization and dynamics of guest proteins in liquid droplets.
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