Evidence map›Paper›PMID 34586910›Full record

ArticleApplied and environmental microbiology2021

A High-Throughput Short Sequence Typing Scheme for Serratia marcescens Pure Culture and Environmental DNA.

Thibault Bourdin, Alizée Monnier, Marie-Ève Benoit, Emilie Bédard, Michèle Prévost, Caroline Quach, Eric Déziel, Philippe Constant

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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  6. Article
  7. Review
  8. Article
  9. Article
  10. Intestinal Dominance byMicroorganisms · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Thibault BourdinInstitut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, Laval, Québec, Canada.ORCID 0000-0001-5257-9374
Alizée MonnierInstitut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, Laval, Québec, Canada.
Marie-Ève BenoitDepartment of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec, Canada.
Emilie BédardDepartment of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec, Canada.
Michèle PrévostDepartment of Civil, Geological, and Mining Engineering, Polytechnique Montréal, Montréal, Québec, Canada.
Caroline QuachUniversité de Montréal, Montréal, Québec, Canada.ORCID 0000-0002-1170-9475
Eric DézielInstitut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, Laval, Québec, Canada.ORCID 0000-0002-4609-0115
Philippe ConstantInstitut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, Laval, Québec, Canada.ORCID 0000-0003-2739-2801

Funding

Gouvernement du Canada | Canadian Institutes of Health Research (CIHR) 396313Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (NSERC) 396313
6 · The paper itself

Abstract

Molecular typing methods are used to characterize the relatedness between bacterial isolates involved in infections. These approaches rely mostly on discrete loci or whole-genome sequencing (WGS) analyses of pure cultures. On the other hand, their application to environmental DNA profiling to evaluate epidemiological relatedness among patients and environments has received less attention. We developed a specific, high-throughput short sequence typing (HiSST) method for the opportunistic human pathogen Serratia marcescens. Genes displaying the highest polymorphism were retrieved from the core genome of 60 S. marcescens strains. Bioinformatics analyses showed that use of only three loci (within

Indexed as

Cross InfectionDNA, EnvironmentalBacterial Typing TechniquesDisease OutbreaksHumansInfant, NewbornIntensive Care Units, NeonatalSerratia marcescensDNA, Environmentalenvironmental DNAhealthcare-associated infections (HAI)HiSSTmolecular typingneonatal intensive care unit (NICU)opportunistic pathogenssink environment

Identifiers

PMID34586910
PMCPMC8612290

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.