ArticlePlant molecular biology2022
Acylsugars protect Nicotiana benthamiana against insect herbivory and desiccation.
Article in Plant molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Structurally diverse acylsugars on wild tomatillo fruit surfaces.Horticulture research · 2026Article
- Transient Expression of Bta78 Gene in Nicotiana benthamiana Enhances Whitefly Feeding Activity.Archives of insect biochemistry and physiology · 2026Article
- Evolutionary hijacking of fatty acid biosynthesis drives insecticidal acylsugar diversity in tomato.Nature communications · 2026Article
- The organization of acylsugar biosynthetic enzymes: what is next for metabolic efficiency in plant defense?Journal of experimental botany · 2026Article
- A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis.Journal of experimental botany · 2026Article
- Basic Research on Whitefly Molecular Biology: A Foundation for Innovative Pest Management Strategies.Current issues in molecular biology · 2026Review
- Plant resistance: scientific basis and latest research progress.Frontiers in plant science · 2026Review
- Wearable sensors: in situ detection tools for plant physiological markers and growth environment information.Mikrochimica acta · 2025Review
- A review of factors affecting the success of geminivirus infectious clones.Plant cell reports · 2025Review
- Expression of a multigene mushroom luciferin biosynthesis pathway as a pseudo-polycistron in plants.Scientific reports · 2025Article
- Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism.The New phytologist · 2025Article
- Article
- The Turnip Yellows Virus Capsid Protein Promotes Access of Its Main Aphid Vector Myzus persicae to Phloem Tissues.Plant, cell & environment · 2025Article
- Sticky plants and plant-based glues: potential for pest control.Frontiers in plant science · 2025Review
- Enabling biocontained plant virus transmission studies through establishment of an axenic whitefly (Bemisia tabaci) colony on plant tissue culture.Scientific reports · 2024Article
- Article
- Tomato root specialized metabolites evolved through gene duplication and regulatory divergence within a biosynthetic gene cluster.Science advances · 2024Article
- Horizontally transferred genes as RNA interference targets for aphid and whitefly control.Plant biotechnology journal · 2023Article
- Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components.Plant physiology · 2023Article
- Blurred lines: Primary metabolic machinery coopted for specialized metabolism in tomato trichomes.Plant physiology · 2023Article
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Authors and funding
13 authors.
Funding
Abstract
key messageNicotiana benthamiana acylsugar acyltransferase (ASAT) is required for protection against desiccation and insect herbivory. Knockout mutations provide a new resource for investigation of plant-aphid and plant-whitefly interactions. Nicotiana benthamiana is used extensively as a transient expression platform for functional analysis of genes from other species. Acylsugars, which are produced in the trichomes, are a hypothesized cause of the relatively high insect resistance that is observed in N. benthamiana. We characterized the N. benthamiana acylsugar profile, bioinformatically identified two acylsugar acyltransferase genes, ASAT1 and ASAT2, and used CRISPR/Cas9 mutagenesis to produce acylsugar-deficient plants for investigation of insect resistance and foliar water loss. Whereas asat1 mutations reduced accumulation, asat2 mutations caused almost complete depletion of foliar acylsucroses. Three hemipteran and three lepidopteran herbivores survived, gained weight, and/or reproduced significantly better on asat2 mutants than on wildtype N. benthamiana. Both asat1 and asat2 mutations reduced the water content and increased leaf temperature. Our results demonstrate the specific function of two ASAT proteins in N. benthamiana acylsugar biosynthesis, insect resistance, and desiccation tolerance. The improved growth of aphids and whiteflies on asat2 mutants will facilitate the use of N. benthamiana as a transient expression platform for the functional analysis of insect effectors and resistance genes from other plant species. Similarly, the absence of acylsugars in asat2 mutants will enable analysis of acylsugar biosynthesis genes from other Solanaceae by transient expression.
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