ArticleScience China. Life sciences2022
Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation.
Article in Science China. Life sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Ultra-fast generation of all-female grass carp via transplantation of female germline stem cells into zebrafish.Science China. Life sciences · 2026Article
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.Communications biology · 2026Article
- Cyprinid Juji (Gobiocypris rarus) as a model fish to study germ cell development and gonadal differentiation.Communications biology · 2025Article
- Generation of donor-derived spermatozoa by cross-subfamily transplantation of purple loach (Leptobotia taeniops) spermatogonia into triploid loach (Misgurnus anguillicaudatus).Fish physiology and biochemistry · 2025Article
- Establishment and Characteristics of the Spermatogonial Stem Cell Line from the Yellow River Carp (Biology · 2025Article
- Article
- A toast to a zebrafish germ cell induction cocktail.Science China. Life sciences · 2024Article
- Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors.Nature communications · 2023Article
- Establishment of aBiology · 2023Article
- Enhancement of zebrafish sperm production via a large body-sized surrogate with germ cell transplantation.Communications biology · 2023Article
- Zebrafish: a convenient tool for myelopoiesis research.Cell regeneration (London, England) · 2023Review
- Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set.Frontiers in endocrinology · 2023Article
- Modified Nucleotides for Chemical and Enzymatic Synthesis of Therapeutic RNA.Current medicinal chemistry · 2023Article
- Germline stem cells in human.Signal transduction and targeted therapy · 2022Review
- Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish,Cells · 2022Article
- Article
- Isolation and Characterization of Germline Stem Cells in Protogynous HermaphroditicInternational journal of molecular sciences · 2022Article
- Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Frontiers in cell and developmental biology · 2021Article
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11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The surrogate reproduction technique, such as inter-specific spermatogonial stem cells (SSCs) transplantation (SSCT), provides a powerful tool for production of gametes derived from endangered species or those with desirable traits. However, generation of genome-edited gametes from a different species or production of gametes from a phylogenetically distant species such as from a different subfamily, by SSCT, has not succeeded. Here, using two small cyprinid fishes from different subfamilies, Chinese rare minnow (gobiocypris rarus, for brief: Gr) and zebrafish (danio rerio), we successfully obtained Gr-derived genome-edited sperm in zebrafish by an optimized SSCT procedure. The transplanted Gr SSCs supported the host gonadal development and underwent normal spermatogenesis, resulting in a reconstructed fertile testis containing Gr spermatids and zebrafish testicular somatic cells. Interestingly, the surrogate spermatozoa resembled those of host zebrafish but not donor Gr in morphology and swimming behavior. When pou5f3 and chd knockout Gr SSCs were transplanted, Gr-derived genome-edited sperm was successfully produced in zebrafish. This is the first report demonstrating surrogate production of gametes from a different subfamily by SSCT, and surrogate production of genome-edited gametes from another species as well. This method is feasible to be applied to future breeding of commercial fish and livestock.
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