Evidence map›Paper›PMID 34585169›Full record

ArticleSTAR protocols2021

Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.

Inga Rødahl, James Gotley, Stacey B Andersen, Meihua Yu, Ahmed M Mehdi, Angelika N Christ, Emma E Hamilton-Williams, Ian H Frazer, Samuel W Lukowski, Janin Chandra

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Cellular Assays to Monitor Antigen Cross-presentation In Vitro.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  3. PD-1 expressing islet-specific CD4Immunology and cell biology · 2025
    Article
  4. Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Inga RødahlCenter for Infectious Medicine, Department of Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden.
James GotleyThe University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
Stacey B AndersenThe Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia.
Meihua YuThe University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
Ahmed M MehdiThe University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
Angelika N ChristThe Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia.
Emma E Hamilton-WilliamsThe University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
Ian H FrazerThe University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
Samuel W LukowskiThe Institute for Molecular Bioscience, The University of Queensland, Woolloongabba, QLD 4067, Australia.
Janin ChandraThe University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD 4102, Australia.
The University of Queensland · AUKarolinska University Hospital · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).

Indexed as

Gene Expression ProfilingMyeloid CellsAnimalsFlow CytometryMembrane ProteinsMiceMicroarray AnalysisMembrane ProteinsCell isolationFlow Cytometry/Mass CytometryHealth SciencesHigh Throughput ScreeningImmunologyMolecular BiologySequence analysisSequencingSingle Cell

Identifiers

PMID34585169
PMCPMC8456112
OpenAlexW3200925214

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.