Evidence map›Paper›PMID 34585157›Full record

ArticleSTAR protocols2021

Denaturing cross-linking immunoprecipitation to identify footprints for RNA-binding proteins.

Michael Rosenberg, Vered Levy, Verena K Maier, Barry Kesner, Roy Blum, Jeannie T Lee

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Michael RosenbergDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Vered LevyDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Verena K MaierDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Barry KesnerDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Roy BlumDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Jeannie T LeeDepartment of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

Funding

Spreading of Xist RNA and Polycomb complexes along the inactive X-chromosome.R01HD097665 · NICHD · MASSACHUSETTS GENERAL HOSPITAL · PI LEE, JEANNIE T · 2019 to 2023
$3.5M
Howard Hughes Medical InstituteNICHD NIH HHS R01 HD097665
6 · The paper itself

Abstract

The isolation of protein-RNA complexes in the "denaturing cross-linked RNA immunoprecipitation" (dCLIP) protocol is based on biotin-tagging proteins of interest, UV cross-linking RNA to protein in vivo, RNase protection assay, and isolating RNA-protein complexes under denaturing conditions over a streptavidin column. Insofar as conventional antibody-based CLIP assays have been challenging to apply to Polycomb complexes, dCLIP has been applied successfully and yields small RNA footprints from which de novo motif analysis can be performed to identify RNA binding motifs. For complete details on the use and execution of this protocol, please refer to Rosenberg et al. (2017).

Indexed as

RNARNA-Binding ProteinsAnimalsEmbryonic Stem CellsHEK293 CellsHumansImmunoprecipitationMiceProtein FootprintingSequence Analysis, RNARNARNA-Binding ProteinsGeneticsGenomicsMolecular BiologyMolecular/Chemical ProbesProtein BiochemistrySystems biology

Identifiers

PMID34585157
PMCPMC8452891

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.