Evidence map›Paper›PMID 34585153›Full record

ArticleSTAR protocols2021

Screening of CRISPR-Cas9-generated point mutant mice using MiSeq and locked nucleic acid probe PCR.

Kommireddy Vasu, Paul L Fox

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Kommireddy VasuDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Paul L FoxDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Cleveland Clinic Lerner College of Medicine · US

Funding

Structural Determinants Of Ceruloplasmin Inflammatory ActivityP01HL076491 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI PLOW, EDWARD FRANKLIN · 2004 to 2019
$27.3M
Adipokines, Aging, and Alzheimers DiseaseR01AG067146 · NIA · CLEVELAND CLINIC LERNER COM-CWRU · PI FOX, PAUL L · 2020 to 2024
$2.4M
Multisite phosphorylated S6K1 directs a regulatory module determining adipocyte lipid metabolismR01DK123236 · NIDDK · CLEVELAND CLINIC LERNER COM-CWRU · PI FOX, PAUL L · 2020 to 2022
$1.4M
NHLBI NIH HHS P01 HL076491NIA NIH HHS R01 AG067146NIDDK NIH HHS R01 DK123236
6 · The paper itself

Abstract

CRISPR-Cas9-mediated, site-directed mutagenesis in mice generates mosaic founder mice with varied efficiency of desired point mutation and other non-homologous end-joined variants. Here, we present a protocol for design, sample preparation, and analysis for identification of mice with the desired mutation. Deep sequencing provides the proportion of reads of a particular allele for each mouse line. Locked nucleic acid probe-based qPCR provides rapid identification of the mutant allele and can be used for genotyping offspring during subsequent breeding for colony establishment. For complete details on the use and execution of this protocol, please refer to Vasu et al. (2021).

Indexed as

AnimalsCRISPR-Cas SystemsDNA Mutational AnalysisFemaleGene EditingHigh-Throughput Nucleotide SequencingMaleMiceMutagenesis, Site-DirectedMutationOligonucleotideslocked nucleic acidOligonucleotidesCRISPRModel organismsMolecular biologySequencing

Identifiers

PMID34585153
PMCPMC8456059
OpenAlexW3199901403

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.