ArticleSTAR protocols2021
Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed.
- Endothelial SLC25A13 links glutamine metabolism to angiogenesis.EMBO molecular medicine · 2026Article
- Protocol for isolation of the brain vasculature from the mouse cortex for proteomics.STAR protocols · 2026Article
- Programming Brain Cell-Type-Selective Delivery In Vivo with Transporter-Guided Therapeutics.bioRxiv : the preprint server for biology · 2026Article
- From brown to white: Brown adipose tissue endothelial cells whiten in culture conditions.Molecular metabolism · 2026Article
- Endothelial cell responses in sepsis are attenuated by targeting truncated procalcitonin.Nature communications · 2026Article
- Stress-induced mitochondrial fragmentation in endothelial cells disrupts blood-retinal barrier integrity causing neurodegeneration.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Article
- Bioinstructive scaffolds enhance stem cell engraftment for functional tissue regeneration.Nature materials · 2025Article
- APJ regulates the balance between self-renewal and differentiation of vascular endothelial stem cells.Inflammation and regeneration · 2025Article
- Primary Mouse Choroidal Endothelial Cell Culture.Bio-protocol · 2025Article
- PRMT1 Ablation in Endothelial Cells Causes Endothelial Dysfunction and Aggravates COPD Attributable to Dysregulated NF-κB Signaling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Endothelial FOXM1 and Dab2 promote diabetic wound healing.JCI insight · 2025Article
- Cell manufacturing for cell-based tissue engineering: a focus on vascularized, skeletal muscle regeneration.Frontiers in chemical engineering · 2025Article
- Mitochondria-containing extracellular vesicles from mouse vs. human brain endothelial cells for ischemic stroke therapy.Journal of controlled release : official journal of the Controlled Release Society · 2024Article
- Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses.Heliyon · 2024Article
- N6-methyladenosine modification of KLF2 may contribute to endothelial-to-mesenchymal transition in pulmonary hypertension.Cellular & molecular biology letters · 2024Article
- The Cavin-1/Caveolin-1 interaction attenuates BMP/Smad signaling in pulmonary hypertension by interfering with BMPR2/Caveolin-1 binding.Communications biology · 2024Article
- Endothelial ERα promotes glucose tolerance by enhancing endothelial insulin transport to skeletal muscle.Nature communications · 2023Article
- Endothelial cell CD36 regulates membrane ceramide formation, exosome fatty acid transfer and circulating fatty acid levels.Nature communications · 2023Article
- Chronic social defeat alters brain vascular-associated cell gene expression patterns leading to vascular dysfunction and immune system activation.Journal of neuroinflammation · 2023Article
- Prioritization and functional validation of target genes from single-cell transcriptomics studies.Communications biology · 2023Article
Corrections and comments
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Authors and funding
24 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endothelial cells (ECs) harbor distinct phenotypical and functional characteristics depending on their tissue localization and contribute to brain, eye, lung, and muscle diseases such as dementia, macular degeneration, pulmonary hypertension, and sarcopenia. To study their function, isolation of pure ECs in high quantities is crucial. Here, we describe protocols for rapid and reproducible blood vessel EC purification established for scRNA sequencing from murine tissues using mechanical and enzymatic digestion followed by magnetic and fluorescence-activated cell sorting. For complete details on the use and execution of these protocol, please refer to Kalucka et al. (2020), Rohlenova et al. (2020), and Goveia et al. (2020).
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