ArticleThe Science of the total environment2022
DNA metabarcoding using nrITS2 provides highly qualitative and quantitative results for airborne pollen monitoring.
Article in The Science of the total environment, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Method comparison of microscopy, metabarcoding, and multispectral imaging flow cytometry for identification and relative abundance analysis of insect-dispersed pollen.Scientific reports · 2026Article
- Tracing the botanical origins of UK heather honey by relative quantification of plant DNA.NPJ science of food · 2025Article
- Authentication of the Botanical Origin of Honey:Journal of agricultural and food chemistry · 2025Article
- Landscape-scale variation in the canopy mycobiome in temperate beech and spruce forest stands explained by leaf water content and elevation.European journal of forest research · 2025Article
- Effect of forest structural attributes on soil microbial diversity in mixed temperate forests.Plant and soil · 2025Article
- Phenological Analysis of Grasses (Poaceae) in Comparison with Aerobiological Data in Moscow (Russia).Plants (Basel, Switzerland) · 2024Article
- Forest top canopy bacterial communities are influenced by elevation and host tree traits.Environmental microbiome · 2024Article
- Plants, pollinators and their interactions under global ecological change: The role of pollen DNA metabarcoding.Molecular ecology · 2023Article
- Impact of climate change on allergic diseases in Germany.Journal of health monitoring · 2023Article
- DNA from herbs can be obtained from air and authenticated by polymerase chain reaction.Heliyon · 2023Article
- Aerobiological Monitoring and Metabarcoding of Grass Pollen.Plants (Basel, Switzerland) · 2023Article
- Investigating the use of pollen DNA metabarcoding to quantify bee foraging and effects of threshold selection.PloS one · 2023Article
- Horizon scan of DNA-based methods for quality control and monitoring of herbal preparations.Frontiers in pharmacology · 2023Review
- Environmental DNA Metabarcoding: A Novel Contrivance for Documenting Terrestrial Biodiversity.Biology · 2022Review
- Detecting Airborne Pollen Using an Automatic, Real-Time Monitoring System: Evidence from Two Sites.International journal of environmental research and public health · 2022Article
- Further reduction in soil bacterial diversity under severe acidification in European temperate forests.European journal of soil scienceArticle
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Airborne pollen monitoring is of global socio-economic importance as it provides information on presence and prevalence of allergenic pollen in ambient air. Traditionally, this task has been performed by microscopic investigation, but novel techniques are being developed to automate this process. Among these, DNA metabarcoding has the highest potential of increasing the taxonomic resolution, but uncertainty exists about whether the results can be used to quantify pollen abundance. In this study, it is shown that DNA metabarcoding using trnL and nrITS2 provides highly improved taxonomic resolution for pollen from aerobiological samples from the Netherlands. A total of 168 species from 143 genera and 56 plant families were detected, while using a microscope only 23 genera and 22 plant families were identified. NrITS2 produced almost double the number of OTUs and a much higher percentage of identifications to species level (80.1%) than trnL (27.6%). Furthermore, regressing relative read abundances against the relative abundances of microscopically obtained pollen concentrations showed a better correlation for nrITS2 (R
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