Evidence map›Paper›PMID 34582785›Full record

ArticleNeuron2021

Single-nucleus transcriptome analysis reveals cell-type-specific molecular signatures across reward circuitry in the human brain.

Matthew N Tran, Kristen R Maynard, Abby Spangler, Louise A Huuki, Kelsey D Montgomery, Vijay Sadashivaiah, Madhavi Tippani, Brianna K Barry, Dana B Hancock, Stephanie C Hicks and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
152citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

152 citing papers in PubMed, 3 syntheses or guidelines pooled it.

  1. Pooled it
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  3. Pooled it
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  12. Fine mapping of PTSD GWAS reveals a role for amygdalabioRxiv : the preprint server for biology · 2026
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92 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Matthew N TranLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA; McKusick-Nathans Institute, Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Kristen R MaynardLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Abby SpanglerLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Louise A HuukiLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Kelsey D MontgomeryLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Vijay SadashivaiahLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Madhavi TippaniLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Brianna K BarryLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Dana B HancockGenOmics, Bioinformatics, and Translational Research Center, Biostatistics and Epidemiology Division, RTI International, Research Triangle Park, NC, USA.
Stephanie C HicksDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Joel E KleinmanLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Thomas M HydeLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Leonardo Collado-TorresLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.
Andrew E JaffeLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA; McKusick-Nathans Institute, Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Mental Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA. Electronic address: andrew.jaffe@libd.org.
Keri MartinowichLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA. Electronic address: keri.martinowich@libd.org.

Funding

Integrating Epigenomics in Human Brain and Genomics of Nicotine DependenceR01DA042090 · NIDA · RESEARCH TRIANGLE INSTITUTE · PI HANCOCK, DANA B · 2016 to 2020
$3.5M
Integrative cellular deconvolution of human brain RNA sequencing dataR01MH123183 · NIMH · LIEBER INSTITUTE, INC. · PI HICKS, STEPHANIE CARINNE, MAYNARD, KRISTEN ROSE · 2020 to 2023
$2.3M
Statistical Methods for the Normalization and Quantification of Single-Cell RNA-Sequencing DataR00HG009007 · NHGRI · JOHNS HOPKINS UNIVERSITY · PI HICKS, STEPHANIE CARINNE · 2018 to 2020
$725k
NHGRI NIH HHS R00 HG009007NIDA NIH HHS R01 DA042090NIMH NIH HHS R01 MH123183
6 · The paper itself

Abstract

Single-cell gene expression technologies are powerful tools to study cell types in the human brain, but efforts have largely focused on cortical brain regions. We therefore created a single-nucleus RNA-sequencing resource of 70,615 high-quality nuclei to generate a molecular taxonomy of cell types across five human brain regions that serve as key nodes of the human brain reward circuitry: nucleus accumbens, amygdala, subgenual anterior cingulate cortex, hippocampus, and dorsolateral prefrontal cortex. We first identified novel subpopulations of interneurons and medium spiny neurons (MSNs) in the nucleus accumbens and further characterized robust GABAergic inhibitory cell populations in the amygdala. Joint analyses across the 107 reported cell classes revealed cell-type substructure and unique patterns of transcriptomic dynamics. We identified discrete subpopulations of D1- and D2-expressing MSNs in the nucleus accumbens to which we mapped cell-type-specific enrichment for genetic risk associated with both psychiatric disease and addiction.

Indexed as

Gene Expression ProfilingRewardBrainBrain MappingCell Nucleusgamma-Aminobutyric AcidGenome-Wide Association StudyHigh-Throughput Nucleotide SequencingHumansInterneuronsMental DisordersNerve NetNeuronsSequence Analysis, RNASubstance-Related Disordersgamma-Aminobutyric Acidaddictionbraingenomicsneurosciencepsychiatryrewardsingle-celltranscriptomics

Identifiers

PMID34582785
PMCPMC8564763

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.