ArticleCell host & microbe2021
Viral recombination systems limit CRISPR-Cas targeting through the generation of escape mutations.
Article in Cell host & microbe, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Gabija restricts phage circularization and DNA replication.Cell host & microbe · 2026Article
- Engineered phages evade the complete defense repertoire of highly phage-resistant MRSA clinical isolates.bioRxiv : the preprint server for biology · 2026Article
- It's not me, it's you: Anti-phage nuclease specificity inside a bacterium.PLoS pathogens · 2026Article
- Mycobacterial non-homologous end joining is required for antiphage defense.Nucleic acids research · 2026Article
- The application of CRISPR gene-editing technology in influenza prevention and control.Frontiers in genome editing · 2026Review
- Rapid CRISPR-Cas9 target-strand nicking can provide phage resistance by reducing DNA abundance.Nucleic acids research · 2025Article
- Gabija restricts phages that antagonize a conserved host DNA repair complex.bioRxiv : the preprint server for biology · 2025Article
- A minimal model of panimmunity maintenance by horizontal gene transfer in the ecological dynamics of bacteria and phages.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- From resistance to remedy: the role of clustered regularly interspaced short palindromic repeats system in combating antimicrobial resistance-a review.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- SspA is a transcriptional regulator of CRISPR adaptation in E. coli.Nucleic acids research · 2025Article
- Assessing the Role of Bacterial Innate and Adaptive Immunity as Barriers to Conjugative Plasmids.Molecular biology and evolution · 2024Article
- CRISPR-Cas12a exhibits metal-dependent specificity switching.Nucleic acids research · 2024Article
- Increasing CRISPR/Cas9-mediated gene editing efficiency in T7 phage by reducing the escape rate based on insight into the survival mechanism.Acta biochimica et biophysica Sinica · 2024Article
- Inhibitors of bacterial immune systems: discovery, mechanisms and applications.Nature reviews. Genetics · 2024Review
- CRISPR-Cas12a exhibits metal-dependent specificity switching.bioRxiv : the preprint server for biology · 2024Article
- CRISPR-Cas-Based Antimicrobials: Design, Challenges, and Bacterial Mechanisms of Resistance.ACS infectious diseases · 2023Review
- CRISPR-Cas effector specificity and cleavage site determine phage escape outcomes.PLoS biology · 2023Article
- Anti-CRISPR Discovery: Using Magnets to Find Needles in Haystacks.Journal of molecular biology · 2023Review
- Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing.Nature microbiology · 2022Article
- Goodbye PAM: Phage λ's Red recombination system cripples PAMs and helps dodge CRISPR attacks.Cell host & microbe · 2021Article
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5 authors at 1 institution in 1 country.
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Abstract
CRISPR-Cas systems provide immunity to bacteria by programing Cas nucleases with RNA guides that recognize and cleave infecting viral genomes. Bacteria and their viruses each encode recombination systems that could repair the cleaved viral DNA. However, it is unknown whether and how these systems can affect CRISPR immunity. Bacteriophage λ uses the Red system (gam-exo-bet) to promote recombination between related phages. Here, we show that λ Red also mediates evasion of CRISPR-Cas targeting. Gam inhibits the host E. coli RecBCD recombination system, allowing recombination and repair of the cleaved DNA by phage Exo-Beta, which promotes the generation of mutations within the CRISPR target sequence. Red recombination is strikingly more efficient than the host's RecBCD-RecA in the production of large numbers of phages that escape CRISPR targeting. These results reveal a role for Red-like systems in the protection of bacteriophages against sequence-specific nucleases, which may facilitate their spread across viral genomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.