ArticleProceedings of the National Academy of Sciences of the United States of America1986
Spreading of human neutrophils is immediately preceded by a large increase in cytoplasmic free calcium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 121 citations in OpenAlex.
- STIM1/2 maintain signaling competence at ER-PM contact sites during neutrophil spreading.The Journal of cell biology · 2025Article
- FNDC4 alleviates cardiac ischemia/reperfusion injury through facilitating HIF1α-dependent cardiomyocyte survival and angiogenesis in male mice.Nature communications · 2024Article
- Localisation of Intracellular Signals and Responses during Phagocytosis.International journal of molecular sciences · 2023Review
- The voltage-gated potassium channel KV1.3 regulates neutrophil recruitment during inflammation.Cardiovascular research · 2022Article
- Treatment with senicapoc in a porcine model of acute respiratory distress syndrome.Intensive care medicine experimental · 2021Article
- Neutrophil signaling during myocardial infarction wound repair.Cellular signalling · 2021Review
- Neutrophil Cell Shape Change: Mechanism and Signalling during Cell Spreading and Phagocytosis.International journal of molecular sciences · 2019Review
- Calcium signalling and related ion channels in neutrophil recruitment and function.European journal of clinical investigation · 2018Review
- Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading.Scientific reports · 2017Article
- The role of STIM and ORAI proteins in phagocytic immune cells.American journal of physiology. Cell physiology · 2016Review
- Ca²⁺ and calpain control membrane expansion during the rapid cell spreading of neutrophils.Journal of cell science · 2013Article
- Cell surface topology creates high Ca2+ signalling microdomains.Cell calcium · 2010Article
- Article
- How cells tiptoe on adhesive surfaces before sticking.Biophysical journal · 2008Article
- Calcium-sensing soluble adenylyl cyclase mediates TNF signal transduction in human neutrophils.The Journal of experimental medicine · 2005Article
- Cytosolic free Ca(2+) changes and calpain activation are required for beta integrin-accelerated phagocytosis by human neutrophils.The Journal of cell biology · 2002Article
- Membrane-proximal calcium transients in stimulated neutrophils detected by total internal reflection fluorescence.Biophysical journal · 1996Article
- Intracellular calcium levels correlate with speed and persistent forward motion in migrating neutrophils.Biophysical journal · 1995Article
- Ca2+ oscillations in neutrophils triggered by immune complexes result from Ca2+ influx.Immunology · 1994Article
- Inflammatory roles of P-selectin.The Journal of clinical investigation · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
When human polymorphonuclear leukocytes (PMN) are placed on various surfaces, they attach and spread rapidly, increasing their diameter severalfold. The spreading is associated with extensive changes in the cytoskeleton. Since many cytoskeletal events are regulated by Ca2+, we measured the cytosolic free calcium concentration ([Ca2+]i) in individual human PMN as they spread. [Ca2+]i was measured in single cells by microspectrofluorometry using the fluorescent Ca2+-sensitive dye fura-2. Immediately before spreading, PMN exhibit a rapid increase in [Ca2+]i, from 69 +/- 51 nM to 547 +/- 190 nM (mean +/- SD, n = 12). [Ca2+]i returns to near resting levels during the next minute, as the cells spread. Neither the spreading nor the [Ca2+]i spike is blocked by removal of extracellular calcium, by verapamil, by calmodulin antagonists, or by mitochondrial or microtubule poisons. Spreading, but not the [Ca2+]i increase, is blocked by the microfilament inhibitor cytochalasin B. Both spreading and the [Ca2+]i spike are blocked by ATP depletion and reversibly blocked by placing the cells in medium containing hypertonic sucrose or sodium chloride. These data strongly suggest that an increase in [Ca2+]i, derived from nonmitochondrial intracellular pools, plays an important role in the microfilament-mediated process of PMN spreading.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.