ArticleProceedings of the National Academy of Sciences of the United States of America1986
Cloning of a portion of the chromosomal gene for human erythrocyte alpha-spectrin by using a synthetic gene fragment.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The spectrin-ankyrin-4.1-adducin membrane skeleton: adapting eukaryotic cells to the demands of animal life.Protoplasma · 2010Review
- Genomic anatomy of a premier major histocompatibility complex paralogous region on chromosome 1q21-q22.Genome research · 2001Article
- Fine mapping of the human pentraxin gene region on chromosome 1q23.Immunogenetics · 1996Article
- Retroposition in a family of carcinoma-associated antigen genes.Molecular and cellular biology · 1993Article
- Alpha I/65 hereditary elliptocytosis in southern Italy: evidence for an African origin.Human genetics · 1992Article
- Four different mutations in codon 28 of alpha spectrin are associated with structurally and functionally abnormal spectrin alpha I/74 in hereditary elliptocytosis.The Journal of clinical investigation · 1991Article
- Evidence that expression of Sp alpha I/65 hereditary elliptocytosis is compounded by a genetic factor that is linked to the homologous alpha-spectrin allele.Human genetics · 1990Article
- Point mutation in the beta-spectrin gene associated with alpha I/74 hereditary elliptocytosis. Implications for the mechanism of spectrin dimer self-association.The Journal of clinical investigation · 1990Article
- Two elliptocytogenic alpha I/74 variants of the spectrin alpha I domain. Spectrin Culoz (GGT----GTT; alpha I 40 Gly----Val) and spectrin Lyon (CTT----TTT; alpha I 43 Leu---Phe).The Journal of clinical investigation · 1990Article
- Novel families of interspersed repetitive elements from the human genome.Nucleic acids research · 1990Article
- Sequence and exon-intron organization of the DNA encoding the alpha I domain of human spectrin. Application to the study of mutations causing hereditary elliptocytosis.The Journal of clinical investigation · 1989Article
- Glucocorticoids modulate mRNA levels for G-protein beta-subunits.The Biochemical journal · 1989Article
- Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Regulation of erythroid cell-specific gene expression during erythropoiesis.The British journal of cancer. Supplement · 1988Review
- Selective expression of an erythroid-specific isoform of protein 4.1.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- BIGPROBE: a computer program that predicts the sequence of long oligonucleotide probes with high reliability.Nucleic acids research · 1988Article
- The Duffy blood group is linked to the alpha-spectrin locus in a large pedigree with autosomal dominant inheritance of Charcot-Marie-Tooth disease type 1.Human genetics · 1988Article
- Isolation and characterization of cDNA clones for human erythrocyte beta-spectrin.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Three RFLPs are detected by an alpha spectrin genomic clone.Nucleic acids research · 1987Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A region of minimal codon degeneracy was selected from the amino acid sequence of the amino-terminal alpha I domain of human erythrocyte spectrin to design a 90-base-pair DNA probe for the screening of a human genomic library. Five complementary oligonucleotides were assembled to form a full-length double-stranded DNA, which was then cloned in an M13 phage vector to generate hybridization probes. Under stringent conditions, a single hybridizing clone was isolated from a total human genomic library. Partial DNA sequence analysis established the 16.8-kilobase-pair isolate as erythrocyte alpha-spectrin by correlation to a known sequence of 131 amino acids. The spectrin 106 amino acid repeat segment is encoded by multiple exons separated by introns of various sizes. Of the 3074 base pairs of DNA sequenced thus far, 12.8% code for amino acids.
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