ArticleLife science alliance2021
Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells.
Article in Life science alliance, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Midzone bundles of the mammalian anaphase spindle are mechanically coupled both locally and globally.bioRxiv : the preprint server for biology · 2026Article
- KIF2A stabilizes intercellular bridge microtubules to maintain mouse embryonic stem cell cytokinesis.The Journal of cell biology · 2025Article
- Molecular design principles for bipolar spindle organization by two opposing motors.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Regulation of minimal spindle midzone organization by mitotic kinases.Nature communications · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.The Journal of cell biology · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.bioRxiv : the preprint server for biology · 2023Article
- Lateral and longitudinal compaction of PRC1 overlap zones drives stabilization of interzonal microtubules.Molecular biology of the cell · 2023Article
- Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly.Nature communications · 2022Article
- Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers.eLife · 2022Article
- Coupling of microtubule bundles isolates them from local disruptions to set the structural stability of the anaphase spindle.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
During mitosis, the spindle undergoes morphological and dynamic changes. It reorganizes at the onset of the anaphase when the antiparallel bundler PRC1 accumulates and recruits central spindle proteins to the midzone. Little is known about how the dynamic properties of the central spindle change during its morphological changes in human cells. Using gene editing, we generated human cells that express from their endogenous locus fluorescent PRC1 and EB1 to quantify their native spindle distribution and binding/unbinding turnover. EB1 plus end tracking revealed a general slowdown of microtubule growth, whereas PRC1, similar to its yeast orthologue Ase1, binds increasingly strongly to compacting antiparallel microtubule overlaps. KIF4A and CLASP1 bind more dynamically to the central spindle, but also show slowing down turnover. These results show that the central spindle gradually becomes more stable during mitosis, in agreement with a recent "bundling, sliding, and compaction" model of antiparallel midzone bundle formation in the central spindle during late mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.