Evidence map›Paper›PMID 34579422›Full record

ReviewPlants (Basel, Switzerland)2021

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants.

Min-Rui Wang, Wenlu Bi, Mukund R Shukla, Li Ren, Zhibo Hamborg, Dag-Ragnar Blystad, Praveen K Saxena, Qiao-Chun Wang

Abstract readReview
In one paragraph

Review in Plants (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Review
  3. Strategies for the Conservation of Plant Genetic Resources.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
  4. Genetic Stability and Photosystem II Functioning of In Vitro-RecoveredInternational journal of molecular sciences · 2025
    Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Comparative Studies for Cryopreservation ofPlants (Basel, Switzerland) · 2024
    Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Min-Rui WangState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling District, Xianyang 712100, China.
Wenlu BiDepartment of Plant Agriculture, Gosling Research Institute for Plant Preservation, University of Guelph, Guelph, ON N1G 2W1, Canada.ORCID 0000-0001-8852-5298
Mukund R ShuklaDepartment of Plant Agriculture, Gosling Research Institute for Plant Preservation, University of Guelph, Guelph, ON N1G 2W1, Canada.ORCID 0000-0002-3580-3538
Li RenInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Zhibo HamborgDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO), 1431 Ås, Norway.ORCID 0000-0001-7070-6621
Dag-Ragnar BlystadDivision of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO), 1431 Ås, Norway.
Praveen K SaxenaDepartment of Plant Agriculture, Gosling Research Institute for Plant Preservation, University of Guelph, Guelph, ON N1G 2W1, Canada.ORCID 0000-0002-7203-6868
Qiao-Chun WangState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling District, Xianyang 712100, China.

Funding

National Key R&D Programe of China 2019YFD1001803National Natural Science Foundation of China 31870686Norges Forskningsråd 255032/E50
6 · The paper itself

Abstract

Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

Indexed as

cryopreservation(epi)genetic integrityfield performancemetabolic stabilityreactive oxygen speciesshoot tipstissue culture

Identifiers

PMID34579422
PMCPMC8467502

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.