ArticleViruses2021
Wastewater-Based Epidemiology and Long-Read Sequencing to Identify Enterovirus Circulation in Three Municipalities in Maricopa County, Arizona, Southwest United States between June and October 2020.
Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Metagenomic analysis and genomic characterization of enterovirus A76 and Norovirus GI.6[P11] co-infection in a patient with acute gastroenteritis in Thailand.Scientific reports · 2025Article
- A 3-Year Environmental Surveillance Reveals Multiple Enteroviruses Including Types A76, A89, A90 and C113 in Eastern China.Food and environmental virology · 2025Article
- Review
- Viruses in Wastewater-A Concern for Public Health and the Environment.Microorganisms · 2024Review
- Intriguing insight into unanswered questions about Mpox: exploring health policy implications and considerations.Health research policy and systems · 2024Article
- Multimodal Surveillance Model for Enterovirus D68 Respiratory Disease and Acute Flaccid Myelitis among Children in Colorado, USA, 2022.Emerging infectious diseases · 2024Review
- Article
- Monkeypox outbreak: Wastewater and environmental surveillance perspective.The Science of the total environment · 2023Review
- Detection of human, porcine and canine picornaviruses in municipal sewage sludge using pan-enterovirus amplicon-based long-read Illumina sequencing.Emerging microbes & infections · 2022Article
- Impact of sample clarification by size exclusion on virus detection and diversity in wastewater-based epidemiology.medRxiv : the preprint server for health sciences · 2022Article
- Canine picornaviruses detected in wastewater in Arizona, USA 2019 and 2021.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2022Article
- Genomic analysis of a recombinant coxsackievirus A19 identified in Xinxiang, China, in 2019.Archives of virology · 2022Article
- Detecting SARS-CoV-2 lineages and mutational load in municipal wastewater and a use-case in the metropolitan area of Thessaloniki, Greece.Scientific reports · 2022Article
- Special Issue "Enteroviruses 2021".Viruses · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We used wastewater-based epidemiology and amplicon-based long-read high-throughput sequencing for surveillance of enteroviruses (EVs) in Maricopa County, Arizona, Southwest United States. We collected 48 samples from 13 sites in three municipalities between 18 June and 1 October 2020, and filtered (175 mL each; 0.45 µm pore size) and extracted RNA from the filter-trapped solids. The RNA was converted to cDNA and processed through two workflows (Sanger sequencing (SSW) and long-read Illumina sequencing (LRISW)) each including a nested polymerase chain reaction (nPCR) assay. We subjected the ~350 bp amplicon from SSW to Sanger sequencing and the ~1900-2400 bp amplicon from LRISW to Illumina sequencing. We identified EV contigs from 11 of the 13 sites and 41.67% (20/48) of screened samples. Using the LRISW, we detected nine EV genotypes from three species (Enterovirus A (CVA4, EV-A76, EV-A90), Enterovirus B (E14) and Enterovirus C (CVA1, CVA11, CVA13, CVA19 and CVA24)) with Enterovirus C representing approximately 90% of the variants. However, the SSW only detected the five Enterovirus C types. Similarity and phylogenetic analysis showed that multiple Enterovirus C lineages were circulating, co-infecting and recombining in the population during the season despite the SARS-CoV-2 pandemic and the non-pharmaceutical public health measures taken to curb transmission.
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