Evidence map›Paper›PMID 34576958›Full record

ArticleMolecules (Basel, Switzerland)2021

Exploring the Role of L10 Loop in New Delhi Metallo-β-lactamase (NDM-1): Kinetic and Dynamic Studies.

Alessandra Piccirilli, Emanuele Criscuolo, Fabrizia Brisdelli, Paola Sandra Mercuri, Sabrina Cherubini, Maria Laura De Sciscio, Mauro Maccarrone, Moreno Galleni, Gianfranco Amicosante, Mariagrazia Perilli

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. A Natural Novel Mutation in theMicrobiology spectrum · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Alessandra PiccirilliDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Emanuele CriscuoloDepartment of Experimental Medicine, Tor Vergata University of Rome, Via Montpellier 1, 00121 Rome, Italy.
Fabrizia BrisdelliDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Paola Sandra MercuriMacromolécules Biologiques, Centre d'Ingénierie des Protéines, InBioS, Université de Liège, 4000 Liège, Belgium.
Sabrina CherubiniDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Maria Laura De SciscioDepartment of Medicine, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, 00128 Rome, Italy.
Mauro MaccarroneDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.ORCID 0000-0002-3990-2963
Moreno GalleniMacromolécules Biologiques, Centre d'Ingénierie des Protéines, InBioS, Université de Liège, 4000 Liège, Belgium.
Gianfranco AmicosanteDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Mariagrazia PerilliDepartment of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.ORCID 0000-0001-7370-1455

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Four NDM-1 mutants (L218T, L221T, L269H and L221T/Y229W) were generated in order to investigate the role of leucines positioned in L10 loop. A detailed kinetic analysis stated that these amino acid substitutions modified the hydrolytic profile of NDM-1 against some β-lactams. Significant reduction of k

Indexed as

Amino Acid SubstitutionAnti-Bacterial Agentsbeta-LactamasesCefazolinCefoxitinEnzyme StabilityHydrogen-Ion ConcentrationImipenemKineticsLeucineMeropenemMolecular Dynamics SimulationMutagenesis, Site-DirectedReal-Time Polymerase Chain ReactionSpectrometry, FluorescenceAnti-Bacterial Agentsbeta-lactamase NDM-1beta-LactamasesCefazolinCefoxitinImipenemLeucineMeropenemkinetic studiesmetallo-β-lactamasesmolecular dynamicNDM-1

Identifiers

PMID34576958
PMCPMC8467308

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.