ReviewMicroorganisms2021
Current Applications of Absolute Bacterial Quantification in Microbiome Studies and Decision-Making Regarding Different Biological Questions.
Review in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06787222 (Effectiveness and Safety of Zingiber Officinale Transdermal Patch for Acne Vulgaris; Study of Interleukin 1 and Skin Microbiome), which is not on this map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effectiveness and Safety of Zingiber Officinale Transdermal Patch for Acne Vulgaris; Study of Interleukin 1 and Skin Microbiome
Who cites it
35 citing papers in PubMed.
- Article
- Use of Fecal Sampling for Assessing Probiotic Effects on the Gut Microbiome: A Focused Narrative Review.Cureus · 2026Review
- Multi-region sampling of the human small intestine using an ingestible device.medRxiv : the preprint server for health sciences · 2026Article
- Disentangling host identity and storage time effects on gut microbiota composition in captive migratory birds using absolute and relative quantification.Current zoology · 2026Article
- Tongue-coating microbiome as a predictor of solid tumors: an updated scoping review of clinical studies.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2026Article
- Evaluating preservation effects on honeybee gut microbiota inocula.Microbiology spectrum · 2026Article
- Advancing the study of microbial symbionts of amphibians and reptiles.Frontiers in amphibian and reptile science · 2026Article
- Interpreting UniFrac with absolute abundance: a conceptual and practical guide.ISME communications · 2026Article
- Absolute quantification of prokaryotes in the microbiome by 16S rRNA qPCR or ddPCR.Nature protocols · 2025Review
- Transcriptomic detection of Candidatus Allocryptoplasma (Anaplasmataceae) in Galápagos marine iguanas (Amblyrhynchus cristatus, Iguanidae).Parasites & vectors · 2025Article
- Review
- Heterofermentative Lactic Acid Bacteria Enhance the Aerobic Stability of Sweet Sorghum Silage.Microbial biotechnology · 2025Article
- Ultrafine-Grained Materials With Antibacterial Properties: A Novel Approach to Reducing Spinal Implant-Associated Infections.JOR spine · 2025Article
- Toward the Next Generation of In Silico Modeling of Dynamic Host-Microbiota Interactions in the Skin.JID innovations : skin science from molecules to population health · 2025Review
- Ecosystem Multifunctionality Regulated by Soil Microbial Activity and Indicator Taxa Versus Biodiversity for Industrial Solar Facilities on the Qinghai-Tibet Plateau.Microorganisms · 2025Article
- Hemoperfusion with Seraph-100 in septic patients removes pathogens and improves clinical outcomes.Scientific reports · 2025Article
- Construction of the time since deposition (TsD) model in saliva stains with 16S rRNA full-length sequencing technology and microbial markers.International journal of legal medicine · 2025Article
- The Respiratory Tract Microbiome and Human Health.Microbial biotechnology · 2025Review
- Antibiotic-perturbed microbiota and the role of probiotics.Nature reviews. Gastroenterology & hepatology · 2025Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
High throughput sequencing has emerged as one of the most important techniques for characterizing microbial dynamics and revealing bacteria and host interactions. However, data interpretation using this technique is mainly based on relative abundance and ignores total bacteria load. In certain cases, absolute abundance is more important than compositional relative data, and interpretation of microbiota data based solely on relative abundance can be misleading. The available approaches for absolute quantification are highly diverse and challenging, especially for quantification in differing biological situations, such as distinguishing between live and dead cells, quantification of specific taxa, enumeration of low biomass samples, large sample size feasibility, and the detection of various other cellular features. In this review, we first illustrate the importance of integrating absolute abundance into microbiome data interpretation. Second, we briefly discuss the most widely used cell-based and molecular-based bacterial load quantification methods, including fluorescence spectroscopy, flow cytometry, 16S qPCR, 16S qRT-PCR, ddPCR, and reference spike-in. Last, we present a specific decision-making scheme for absolute quantification methods based on different biological questions and some of the latest quantitative methods and procedure modifications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.