ReviewJournal of fungi (Basel, Switzerland)2021
Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous Taxa.
Review in Journal of fungi (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- Culturable Fungi Recovered from Deep-Sea Sediments: Diversity, Phylogenetic Placement, and In Vitro Antagonistic Activity Against Phytopathogenic Fungi.Journal of fungi (Basel, Switzerland) · 2026Article
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- Comparative Metabarcoding of ITS1, ITS2, and Full-Length ITS Reveals Marker- and Tissue-Specific Variation in Fungal Community Profiling in Potato.Plant-environment interactions (Hoboken, N.J.) · 2026Article
- Taxonomy, phylogeny, and evolutionary diversification of spider-pathogenic fungi from China (IMA fungus · 2026Article
- The molecular revolution in fungal diagnostics: bridging gaps across clinical, agricultural, and environmental mycology.Mycology · 2026Review
- Phytomycobiomes and Ecosystem Services: Mechanisms, Evidence and Routes to Application.Journal of fungi (Basel, Switzerland) · 2025Review
- Article
- Recent Advances and Developments in Bacterial Endophyte Identification and Application: A 20-Year Landscape Review.Plants (Basel, Switzerland) · 2025Review
- Unearthing limestone fungal diversity: Description of seven novel species from Portugal.Fungal systematics and evolution · 2025Article
- Discovering the dynamics of peach fruit mycobiome throughout fruit development season by high-throughput sequencing.Scientific reports · 2025Article
- Fungal diversity in the soil Mycobiome: Implications for ONE health.One health (Amsterdam, Netherlands) · 2024Review
- A comprehensive guide to assess gut mycobiome and its role in pathogenesis and treatment of inflammatory bowel disease.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2024Review
- Diversity of Endophytes ofLife (Basel, Switzerland) · 2024Article
- Variations in the structure and function of the soil fungal communities in the traditional cropping systems from Madeira Island.Frontiers in microbiology · 2024Article
- Culture-based diversity of endophytic fungi of three species ofFrontiers in microbiology · 2024Article
- Article
- Rhodophyta DNA Barcoding: Ribulose-1, 5-Bisphosphate Carboxylase Gene and Novel Universal Primers.International journal of molecular sciences · 2023Article
- OMICS and Other Advanced Technologies in Mycological Applications.Journal of fungi (Basel, Switzerland) · 2023Review
- The Endophytic Fungi Diversity, Community Structure, and Ecological Function Prediction ofMicroorganisms · 2022Article
- Current Insight into Traditional and Modern Methods in Fungal Diversity Estimates.Journal of fungi (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Culture techniques are vital in both traditional and modern fungal taxonomy. Establishing sexual-asexual links and synanamorphs, extracting DNA and secondary metabolites are mainly based on cultures. However, it is widely accepted that a large number of species are not sporulating in nature while others cannot be cultured. Recent ecological studies based on culture-independent methods revealed these unculturable taxa, i.e., dark taxa. Recent fungal diversity estimation studies suggested that environmental sequencing plays a vital role in discovering missing species. However, Sanger sequencing is still the main approach in determining DNA sequences in culturable species. In this paper, we summarize culture-based and culture-independent methods in the study of ascomycetous taxa. High-throughput sequencing of leaf endophytes, leaf litter fungi and fungi in aquatic environments is important to determine dark taxa. Nevertheless, currently, naming dark taxa is not recognized by the ICN, thus provisional naming of them is essential as suggested by several studies.
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