Evidence map›Paper›PMID 34573410›Full record

ArticleGenes2021

Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice.

Federico Anaclerio, Rossella Ferrante, Domitilla Mandatori, Ivana Antonucci, Matteo Capanna, Verena Damiani, Pamela Di Tomo, Roberto Ferrante, Marianna Ranaudo, Vincenzo De Laurenzi and 2 more

Abstract read
In one paragraph

Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Federico AnaclerioCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Rossella FerranteCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Domitilla MandatoriCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Ivana AntonucciCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.ORCID 0000-0002-6594-2272
Matteo CapannaCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Verena DamianiCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.ORCID 0000-0003-2833-841X
Pamela Di TomoCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.ORCID 0000-0003-1995-3306
Roberto FerranteCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Marianna RanaudoCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Vincenzo De LaurenziCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Liborio StuppiaCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Simone De FabritiisCenter for Advanced Studies and Technology (CAST), G.d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A considerable effort has been devoted in all countries to react to the COVID-19 pandemic by tracing infected individuals, containing the spread of the disease, identifying therapies, and producing and distributing vaccines. Currently, a significant concern is the appearance of variants of the virus that may frustrate these efforts by showing increased transmissibility, increased disease severity, reduced response to therapy or vaccines, and ability to escape diagnosis. All countries have therefore devoted a massive attempt to the identification and tracking of these variants, which requires a vast technological effort to sequence a large number of viral genomes. In this paper, we report our experience as one of the Italian laboratories involved in SARS-CoV-2 variant tracing. We summarize the different approaches used, and outline a potential model combining several techniques to increase tracing ability while at the same time minimizing costs.

Indexed as

COVID-19COVID-19 Nucleic Acid TestingGenome, ViralHumansLaboratoriesMutationPandemicsReal-Time Polymerase Chain ReactionRNA, ViralSARS-CoV-2Sequence AnalysisRNA, Virallaboratory practiceNGSqRT-PCRSangerSARS-CoV-2 variantstracking

Identifiers

PMID34573410
PMCPMC8467753

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.