Evidence map›Paper›PMID 34571826›Full record

ArticleCells2021

A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ.

Jacob Lam, Prasanna Katti, Michelle Biete, Margaret Mungai, Salma AshShareef, Kit Neikirk, Edgar Garza Lopez, Zer Vue, Trace A Christensen, Heather K Beasley and 8 more

Abstract read
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 208 papers.

0numbers the graph read from it
0cells of the map it votes in
208citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

208 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Polyphenol-RichMolecules (Basel, Switzerland) · 2026
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. ELABELA Targets Mitochondria to Modulate Heart Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

148 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Jacob LamDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, 375 Newton Rd, Iowa City, IA 52242, USA.
Prasanna KattiNational Heart, Lung and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
Michelle BieteDaniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, 200 West Kawili St, Hilo, HI 96720, USA.
Margaret MungaiDepartment of Molecular and Cell Biology, University of California Berkeley, 142 Weill Hall, Berkeley, CA 94720, USA.
Salma AshShareefDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, 375 Newton Rd, Iowa City, IA 52242, USA.
Kit NeikirkDaniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, 200 West Kawili St, Hilo, HI 96720, USA.
Edgar Garza LopezDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN 37235, USA.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN 37235, USA.ORCID 0000-0002-3502-8906
Trace A ChristensenMicroscopy and Cell Analysis Core Facility, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Heather K BeasleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN 37235, USA.ORCID 0000-0002-4636-2889
Taylor A RodmanDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN 37235, USA.
Sandra A MurrayDepartment of Cell Biology, School of Medicine, University of Pittsburgh, 3550 Terrace St., Pittsburgh, PA 15213, USA.
Jeffrey L SalisburyMicroscopy and Cell Analysis Core Facility, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Brian GlancyNational Heart, Lung and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
Jianqiang ShaoCentral Microscopy Research Facility, University of Iowa, Iowa City, IA 52242, USA.
Renata O PereiraDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, 375 Newton Rd, Iowa City, IA 52242, USA.
E Dale AbelDepartment of Internal Medicine, Carver College of Medicine, University of Iowa, 375 Newton Rd, Iowa City, IA 52242, USA.ORCID 0000-0001-5290-0738
Antentor HintonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, 2201 West End Ave, Nashville, TN 37235, USA.

Funding

INTERDISCIPLINARY CARDIOVASCULAR RESEARCH FELLOWSHIPT32HL007121 · NHLBI · UNIVERSITY OF IOWA · PI LONDON, BARRY · 1985 to 2020
$15.1M
The role of the integrated stress response in brown adipose tissue-mediated metabolic adaptationsR01DK125405 · NIDDK · UNIVERSITY OF IOWA · PI PEREIRA ALAMBERT, RENATA · 2020 to 2024
$1.9M
Insulin Resistance and Myocardial AutophagyR01HL108379 · NHLBI · UNIVERSITY OF UTAH · PI ABEL, E DALE · 2011 to 2014
$1.5M
Insulin Signaling and the Heart in DiabetesR01DK092065 · NIDDK · UNIVERSITY OF UTAH · PI ABEL, E DALE · 2010 to 2011
$649k
NHLBI NIH HHS R01 HL108379NHLBI NIH HHS T32 HL007121NIDDK NIH HHS R01 DK092065NIDDK NIH HHS R01 DK125405
6 · The paper itself

Abstract

Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software

Indexed as

AnimalsCells, CulturedEndoplasmic ReticulumMaleMiceMice, Inbred C57BLMicroscopy, Electron, TransmissionMitochondriaMitochondrial MembranesSoftwarecristaeimage analysisImageJimage processingmitochondriaMitochondria Endoplasmic Reticulum Contacts (MERCs)TEM analysisTEM quantification

Identifiers

PMID34571826
PMCPMC8465115

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.