Evidence map›Paper›PMID 34571353›Full record

ArticleDiagnostic microbiology and infectious disease2021

Use of real-time PCR as an alternative to conventional genotyping methods for the laboratory detection of lymphogranuloma venereum (LGV).

Evonne N Woodson, Samantha S Katz, Sheree S Mosley, Damien C Danavall, Katherine E Bowden, Kai-Hua Chi, Brian H Raphael

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic microbiology and infectious disease, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it, 5 citations in OpenAlex.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 3 countries.

Evonne N WoodsonLaboratory Leadership Service, Centers for Disease Control and Prevention, Atlanta, GA, USA; Division of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: phy2@cdc.gov.
Samantha S KatzDivision of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Sheree S MosleyDivision of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Damien C DanavallDivision of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Katherine E BowdenDivision of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Kai-Hua ChiDivision of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Brian H RaphaelDivision of STD Prevention, National Center for HIV, Hepatitis, STD, TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Centers for Disease Control and Prevention · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

Lymphogranuloma venereum (LGV) can be differentiated from non-LGV chlamydial infection using Sanger sequencing or molecular assays, including those that are commercially-available internationally. Here, we describe the performance of a rapid real-time PCR (RT-PCR)-based strategy in differentiating Chlamydia trachomatis infections associated with LGV or non-LGV serovars. One hundred three rectal swabs, previously genotyped using Sanger sequencing of the ompA gene as a reference method, were tested in the RT-PCR assays. All non-LGV specimens were correctly identified, but the RT-PCR failed to detect 1 LGV specimen, resulting in a sensitivity of 87.5% for the non-LGV/LGV RT-PCR assay. Additional performance characteristics (e.g., specificity, accuracy, and reproducibility) were all between 93% and 100% with a limit of detection ≤100 copies/reaction. Thus, this rapid RT-PCR method for LGV detection in clinical specimens is comparable to the reference method.

Indexed as

Bacterial Outer Membrane ProteinsChlamydia trachomatisDNA, BacterialGenome, BacterialGenotypeHumansLymphogranuloma VenereumMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionRectumReproducibility of ResultsSensitivity and SpecificitySequence Analysis, DNASerogroupBacterial Outer Membrane ProteinsDNA, BacterialOMPA outer membrane proteinsChlamydia trachomatisLymphogranuloma venereumOuter membrane protein A (ompA)Real-time PCR

Identifiers

PMID34571353
PMCPMC8650105
OpenAlexW3197802562

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.