ArticleActa odontologica latinoamericana : AOL2021
Cytotoxic and anti-inflammatory effects of chitosan and hemostatic gelatin in oral cell culture.
Article in Acta odontologica latinoamericana : AOL, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- Frequency-Regulated Repeated Micro-Vibration Promotes Osteoblast Differentiation Through BMP Signaling in MC3T3-E1 Cells.Life (Basel, Switzerland) · 2025Article
- Gelatin-Chitosan Hydrogel Biological, Antimicrobial and Mechanical Properties for Dental Applications.Biomimetics (Basel, Switzerland) · 2023Article
- Chitosan-graphene quantum dot based active film as smart wound dressing.Journal of drug delivery science and technology · 2023Article
- Gelatin-based hemostatic agents for medical and dental application at a glance: A narrative literature review.The Saudi dental journal · 2022Review
- Cytotoxic Potential of Denture Adhesives on Human Fibroblasts-In Vitro Study.Materials (Basel, Switzerland) · 2022Article
- Evaluation of push-out bond strength of different concentrations of chitosan nanoparticles incorporated composite resin and eighth-generation bonding agent for class II restoration: AnJournal of conservative dentistry : JCDArticle
- Effects of mouthwash exposure to stainless steel temporary anchorage device on cytotoxicity and inflammation levels of BHK-21 cells.Journal of advanced pharmaceutical technology & researchArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Chitosan is a biopolymer with bactericidal/bacteriostatic effect, biocompatible and biodegradable. It has been used in tissue engineering to replace tissues partially or completely by releasing bioactive materials or influencing cell growth, usually in regenerative medicine and dentistry. The aim of this study was to evaluate the cytotoxic and anti-inflammatory effect of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF were isolated from patients. Cells were subcultured in DMEM. Chitosan was inoculated at different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) were placed directly in the presence of cells and incubated for 24 hours. Cell viability was determined by MTT method and mean cytotoxic concentration (CC50) was calculated from the dose-response curve. Anti-inflammatory effect was calculated from the in vitro gingivitis model induced with interleukin 1beta (IL-1β) in HGF and protein detection. The data were subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney tests. Experiments were performed in triplicate of three independent assays. Cell viability of HPC, HGF and MC3T3-E1 in contact with chitosan decreased significantly (p<0.05). The HPC were the most sensitive (CC50= 0.18%), followed by HGF (CC50= 0.18%) and MC3T3-E1 (CC50= 0.19%). The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory effect was reduced significantly in the gingivitis model. To conclude, chitosan induces moderate cytotoxic effects alone or with hemostatic gelatin at 0.19%, in dose-dependent manner, with anti-inflammatory effects on human gingival fibroblasts. The use of chitosan as a biomaterial can be an excellent choice for use in regenerative dentistry.
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