ArticleAngewandte Chemie (International ed. in English)2021
Enhanced Ribozyme-Catalyzed Recombination and Oligonucleotide Assembly in Peptide-RNA Condensates.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- The RNA-First Fallacy: Conflating Evolutionary Ancestry with Prebiotic Primacy.Life (Basel, Switzerland) · 2026Article
- Recent advances in coacervate protocells from passive catalysts to chemically programmable systems.Communications chemistry · 2026Review
- Growth, Dissolution and Segregation of Genetically Encoded RNA Droplets by Ribozyme Catalysis.Angewandte Chemie (International ed. in English) · 2026Article
- Complex and Messy Prebiotic Chemistry: Obstacles and Opportunities for an RNA World.Life (Basel, Switzerland) · 2026Review
- Protometabolically Generated NADH Mediates Material Properties of Aqueous Dispersions to Coacervate Microdroplets.Biomacromolecules · 2026Article
- Article
- RNA-peptide interactions tune the ribozyme activity within coacervate microdroplet dispersions.Nature communications · 2025Article
- Exploring the space of self-reproducing ribozymes using generative models.Nature communications · 2025Article
- Aqueous Two-Phase Submicron Droplets Catalyze DNA Nanostructure Assembly for Confined Fluorescent Biosensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst?Chembiochem : a European journal of chemical biology · 2025Review
- Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition.Biomacromolecules · 2025Article
- Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment.Beilstein journal of nanotechnology · 2025Review
- The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability.Nucleic acids research · 2024Article
- How Droplets Can Accelerate Reactions─Coacervate Protocells as Catalytic Microcompartments.Accounts of chemical research · 2024Article
- Fundamental Aspects of Phase-Separated Biomolecular Condensates.Chemical reviews · 2024Review
- Constrained dynamics of DNA oligonucleotides in phase-separated droplets.Biophysical journal · 2024Article
- Physical limits to acceleration of enzymatic reactions inside phase-separated compartments.Physical review. E · 2024Article
- Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates.Communications chemistry · 2024Article
- Nonequilibrium Wet-Dry Cycling Acts as a Catalyst for Chemical Reactions.The journal of physical chemistry. B · 2024Article
- RNA folding studies inside peptide-rich droplets reveal roles of modified nucleosides at the origin of life.Science advances · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The ability of RNA to catalyze RNA ligation is critical to its central role in many prebiotic model scenarios, in particular the copying of information during self-replication. Prebiotically plausible ribozymes formed from short oligonucleotides can catalyze reversible RNA cleavage and ligation reactions, but harsh conditions or unusual scenarios are often required to promote folding and drive the reaction equilibrium towards ligation. Here, we demonstrate that ribozyme activity is greatly enhanced by charge-mediated phase separation with poly-L-lysine, which shifts the reaction equilibrium from cleavage in solution to ligation in peptide-RNA coaggregates and coacervates. This compartmentalization enables robust isothermal RNA assembly over a broad range of conditions, which can be leveraged to assemble long and complex RNAs from short fragments under mild conditions in the absence of exogenous activation chemistry, bridging the gap between pools of short oligomers and functional RNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.