Evidence map›Paper›PMID 34568935›Full record

ArticleG3 (Bethesda, Md.)2021

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research.

Qing Xiao, Huadong Wang, Nuan Song, Zewen Yu, Khan Imran, Weibo Xie, Shuqing Qiu, Fasong Zhou, Jing Wen, Cheng Dai and 5 more

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Sef1, rapid-cycling Brassica napus for large-scale functional genome research in a controlled environment.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023
    Article
  7. BnaC01.BIN2, a GSK3-like kinase, modulates plant height and yield potential in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023
    Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Qing XiaoCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.
Huadong WangCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.ORCID 0000-0001-7665-2046
Nuan SongCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.
Zewen YuCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.
Khan ImranDepartment of Biochemistry, School of Dental Medicine; University of Pennsylvania, Philadelphia, USA 19104-6303.
Weibo XieCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.ORCID 0000-0002-2768-3572
Shuqing QiuGreenfafa Institute of Novel Genechip R&D Co. Ltd., Wuhan, China 430010.
Fasong ZhouGreenfafa Institute of Novel Genechip R&D Co. Ltd., Wuhan, China 430010.
Jing WenCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.
Cheng DaiCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.ORCID 0000-0002-4853-8278
Chaozhi MaCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.
Jinxing TuCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.ORCID 0000-0001-5131-4526
Jinxiong ShenCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.
Tingdong FuCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.
Bin YiCollege of plant science and technology; National Key Laboratory of Crop Genetic Improvement; Huazhong Agricultural University, Wuhan, China, 430070.ORCID 0000-0002-9343-2616

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapeseed is a globally cultivated commercial crop, primarily grown for its oil. High-density single nucleotide polymorphism (SNP) arrays are widely used as a standard genotyping tool for rapeseed research, including for gene mapping, genome-wide association studies, germplasm resource analysis, and cluster analysis. Although considerable rapeseed genome sequencing data have been released, DNA arrays are still an attractive choice for providing additional genetic data in an era of high-throughput whole-genome sequencing. Here, we integrated re-sequencing DNA array data (32,216, 304 SNPs) from 505 inbred rapeseed lines, allowing us to develop a sensitive and efficient genotyping DNA array, Bnapus50K, with a more consistent genetic and physical distribution of probes. A total of 42,090 high-quality probes were filtered and synthesized, with an average distance between adjacent SNPs of 8 kb. To improve the practical application potential of this array in rapeseed breeding, we also added 1,618 functional probes related to important agronomic traits such as oil content, disease resistance, male sterility, and flowering time. The additional probes also included those specifically for detecting genetically modified material. These probes show a good detection efficiency and are therefore useful for gene mapping, along with crop variety improvement and identification. The novel Bnapus50K DNA array developed in this study could prove to be a quick and versatile genotyping tool for B. napus genomic breeding and research.

Indexed as

Brassica napusGenome-Wide Association StudyGenomicsGenotypePlant BreedingPolymorphism, Single NucleotideBnapus50KBrassica napusDNA arraygene mappinggenotyping toolmolecular breedingsingle nucleotide polymorphisms (SNPs)

Identifiers

PMID34568935
PMCPMC8473974

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.