ArticleMicrosystems & nanoengineering2020
Single-cell individualized electroporation with real-time impedance monitoring using a microelectrode array chip.
Article in Microsystems & nanoengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Unraveling the dielectric heterogeneity response of cell lines under electroporation: a microfluidic single-cell on-chip system.Microsystems & nanoengineering · 2026Article
- Microparticle-enabled single cell multiparameter electronic immunophenotyping for selective electroporation.Lab on a chip · 2026Article
- A critical review of microfluidic electroporation for therapeutic cell engineering.Cell reports. Physical science · 2026Article
- Microfluidic Electroporation: Recent Advances and Applications.Advances in experimental medicine and biology · 2026Review
- The Pre-Polarization and Concentration of Cells near Micro-Electrodes Using AC Electric Fields Enhances the Electrical Cell Lysis in a Sessile Drop.Biosensors · 2025Article
- Novel electroporation microchip with field constriction enhances transfection efficiency and survival rates of feline embryos.Scientific reports · 2024Article
- Efficient electroporation in primary cells with PEDOT:PSS electrodes.Science advances · 2024Article
- Microfluidic-Based Electrical Operation and Measurement Methods in Single-Cell Analysis.Sensors (Basel, Switzerland) · 2024Review
- Analysis of In Situ Electroporation Utilizing Induced Electric Field at a Wireless Janus Microelectrode.Micromachines · 2024Article
- Microfluidic Electroporation Arrays for Investigating Electroporation-Induced Cellular Rupture Dynamics.Biosensors · 2024Article
- Recent Advancements in Biomaterials for Chimeric Antigen Receptor T Cell Immunotherapy.Biomaterials research · 2024Review
- A graphene microelectrode array based microfluidic device forNanoscale advances · 2023Article
- Optimization design of interdigitated microelectrodes with an insulation layer on the connection tracks to enhance efficiency of assessment of the cell viability.BMC biomedical engineering · 2023Article
- Highly efficient cell-microbead encapsulation using dielectrophoresis-assisted dual-nanowell array.PNAS nexus · 2023Article
- piRT-IFC: Physics-informed real-time impedance flow cytometry for the characterization of cellular intrinsic electrical properties.Microsystems & nanoengineering · 2023Article
- Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- Intracellular Recording of Cardiomyocytes by Integrated Electrical Signal Recording and Electrical Pulse Regulating System.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to precisely deliver molecules into single cells while maintaining good cell viability is of great importance to applications in therapeutics, diagnostics, and drug delivery as it is an advancement toward the promise of personalized medicine. This paper reports a single-cell individualized electroporation method with real-time impedance monitoring to improve cell perforation efficiency and cell viability using a microelectrode array chip. The microchip contains a plurality of sextupole-electrode units patterned in an array, which are used to perform in situ electroporation and real-time impedance monitoring on single cells. The dynamic recovery processes of single cells under electroporation are tracked in real time via impedance measurement, which provide detailed transient cell states and facilitate understanding the whole recovery process at the level of single cells. We define single-cell impedance indicators to characterize cell perforation efficiency and cell viability, which are used to optimize electroporation. By applying the proposed electroporation method to different cell lines, including human cancer cell lines and normal human cell lines individually, optimum stimuli are determined for these cells, by which high transfection levels of enhanced green fluorescent protein (EGFP) plasmid into cells are achieved. The results validate the effectiveness of the proposed single-cell individualized electroporation/transfection method and demonstrate promising potential in applications of cell reprogramming, induced pluripotent stem cells, adoptive cell therapy, and intracellular drug delivery technology.
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Registered trials
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