ArticleMicrosystems & nanoengineering2020
Oil-mediated high-throughput generation and sorting of water-in-water droplets.
Article in Microsystems & nanoengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Droplet microfluidics for biomedical applications: emerging trends and future developments.Microsystems & nanoengineering · 2026Review
- Construction of Large-Scale Bioengineered Hair Germs and In Vivo Transplantation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Thermo-responsive aqueous two-phase system for two-level compartmentalization.Nature communications · 2024Article
- Droplet-Based Microfluidics: Applications in Pharmaceuticals.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Self-synchronization of reinjected droplets for high-efficiency droplet pairing and merging.Microsystems & nanoengineering · 2023Article
- Progress in all-aqueous droplets generation with microfluidics: Mechanisms of formation and stability improvements.Biophysics reviews · 2022Review
- Material Engineering in Gut Microbiome and Human Health.Research (Washington, D.C.) · 2022Review
- Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Formation of double emulsion micro-droplets in a microfluidic device using a partially hydrophilic-hydrophobic surface.RSC advances · 2021Article
- Simultaneous Droplet Generation with In-Series Droplet T-Junctions Induced by Gravity-Induced Flow.Micromachines · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aqueous two-phase system (ATPS) droplets have demonstrated superior compatibility over conventional water-in-oil droplets for various biological assays. However, the ultralow interfacial tension hampers efficient and stable droplet generation, limiting further development and more extensive use of such approaches. Here, we present a simple strategy to employ oil as a transient medium for ATPS droplet generation. Two methods based on passive flow focusing and active pico-injection are demonstrated to generate water-water-oil double emulsions, achieving a high generation frequency of ~2.4 kHz. Through evaporation of the oil to break the double emulsions, the aqueous core can be released to form uniform-sized water-in-water droplets. Moreover, this technique can be used to fabricate aqueous microgels, and the introduction of the oil medium enables integration of droplet sorting to produce single-cell-laden hydrogels with a harvest rate of over 90%. We believe that the demonstrated high-throughput generation and sorting of ATPS droplets represent an important tool to advance droplet-based tissue engineering and single-cell analyses.
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Registered trials
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