ReviewMicrosystems & nanoengineering2020
How single-cell immunology is benefiting from microfluidic technologies.
Review in Microsystems & nanoengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 75 citations in OpenAlex.
- Nested Nanowell Arrays for High-Throughput Quantitative Analysis of Cytokines from Single Macrophages.ACS nano medicine · 2026Article
- A high-throughput modular microfluidic platform for versatile functional assays at single-cell level.Microsystems & nanoengineering · 2026Article
- Cell-Based Immuno-Biosensors Using Microfluidics.Sensors (Basel, Switzerland) · 2026Review
- Microfluidics for cell therapy and manufacturing in oncology and regenerative medicine.Lab on a chip · 2026Review
- A Facile Method Based on Faster R-CNN for Cell Detection in Microfluidic Devices.Analytical chemistry · 2026Article
- Article
- Multiplexed profiling of single-cell secretion on a hierarchical loading microwell chip.Biosensors & bioelectronics · 2025Article
- Microfluidic Sensors Integrated with Smartphones for Applications in Forensics, Agriculture, and Environmental Monitoring.Micromachines · 2025Review
- In-Line Enrichment of Cancer Cells from Whole Blood by Cell Self-Organization in Acoustic Fields.Analytical chemistry · 2025Article
- Synergistic potential of stem cells and microfluidics in regenerative medicine.Molecular and cellular biochemistry · 2025Review
- Ultrasensitive detection of intact SARS-CoV-2 particles in complex biofluids using microfluidic affinity capture.Science advances · 2025Article
- Organ-on-chip for advancing CAR therapy.Clinical & translational immunology · 2025Review
- Single-cell Technology in Stem Cell Research.Current stem cell research & therapy · 2025Review
- Enhancing the sensitivity of immunoassays toward single-cell secretion analysis.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Microliter Whole Blood Neutrophil Assay Preserving Physiological Lifespan and Functional Heterogeneity.Small methods · 2024Article
- Dielectrophoretic capture and electrochemical enzyme-linked immunosorbent assay of single melanoma cells at an array of interlocked spiral bipolar electrodes.ChemElectroChem · 2024Article
- Microphysiological Systems for Cancer Immunotherapy Research and Development.Advanced biology · 2024Review
- Label-free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing.Scientific reports · 2024Article
- Antibodies, repertoires and microdevices in antibody discovery and characterization.Lab on a chip · 2024Review
- Technologies to Study Genetics and Molecular Pathways.Advances in experimental medicine and biology · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The immune system is a complex network of specialized cells that work in concert to protect against invading pathogens and tissue damage. Imbalances in this network often result in excessive or absent immune responses leading to allergies, autoimmune diseases, and cancer. Many of the mechanisms and their regulation remain poorly understood. Immune cells are highly diverse, and an immune response is the result of a large number of molecular and cellular interactions both in time and space. Conventional bulk methods are often prone to miss important details by returning population-averaged results. There is a need in immunology to measure single cells and to study the dynamic interplay of immune cells with their environment. Advances in the fields of microsystems and microengineering gave rise to the field of microfluidics and its application to biology. Microfluidic systems enable the precise control of small volumes in the femto- to nanoliter range. By controlling device geometries, surface chemistry, and flow behavior, microfluidics can create a precisely defined microenvironment for single-cell studies with spatio-temporal control. These features are highly desirable for single-cell analysis and have made microfluidic devices useful tools for studying complex immune systems. In addition, microfluidic devices can achieve high-throughput measurements, enabling in-depth studies of complex systems. Microfluidics has been used in a large panel of biological applications, ranging from single-cell genomics, cell signaling and dynamics to cell-cell interaction and cell migration studies. In this review, we give an overview of state-of-the-art microfluidic techniques, their application to single-cell immunology, their advantages and drawbacks, and provide an outlook for the future of single-cell technologies in research and medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.