ArticleMicrosystems & nanoengineering2020
3D microgroove electrical impedance sensing to examine 3D cell cultures for antineoplastic drug assessment.
Article in Microsystems & nanoengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Label-Free Electrochemical Impedance Spectroscopy for Biosensing: Evolving Interfaces and Mechanistic Insights.Small science · 2025Review
- Real-Time Monitoring of NIH/3T3 Cell Growth and Drug Reaction Using Impedance Biosensors and Comparison with Biological Assays.Biosensors · 2025Article
- High-throughput impedance monitoring in 3D tumor cultures: a multiplex, microfluidic-free platform for drug screening.Lab on a chip · 2025Article
- 3D In Vitro Models of Breast Cancer: Current Challenges and Future Prospects Toward Recapitulating the Microenvironment and Mimicking Key Processes.Advanced biology · 2025Review
- Quantitative impedance-based characterization of breast cancer cell migration and metastatic potential.Journal of biological engineering · 2025Article
- Bioelectronic Interfaces and Sensors for Neural Organoids.Microsystems & nanoengineering · 2025Review
- A Signal-On Microelectrode Electrochemical Aptamer Sensor Based on AuNPs-MXene for Alpha-Fetoprotein Determination.Sensors (Basel, Switzerland) · 2024Article
- Vertical impedance electrode array for spatiotemporal dynamics monitoring of 3D cells under drug diffusion effect.iScience · 2023Article
- Effect of Anisotropic Structural Depth on Orientation and Differentiation Behavior of Skeletal Muscle Cells.ACS omega · 2023Article
- Improvements in Maturity and Stability of 3D iPSC-Derived Hepatocyte-like Cell Cultures.Cells · 2023Article
- High-Throughput Electromechanical Coupling Chip Systems for Real-Time 3D Invasion/Migration Assay of Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Optimization design of interdigitated microelectrodes with an insulation layer on the connection tracks to enhance efficiency of assessment of the cell viability.BMC biomedical engineering · 2023Article
- Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy.Sensors (Basel, Switzerland) · 2023Article
- A Review on Microfluidics-Based Impedance Biosensors.Biosensors · 2023Review
- Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability.Biosensors · 2022Review
- The Anti-Proliferative Effect of PI3K/mTOR and ERK Inhibition in Monolayer and Three-Dimensional Ovarian Cancer Cell Models.Cancers · 2022Article
- Review
- Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases.Cardiovascular research · 2021Review
- Microfluidic-Chip-Integrated Biosensors for Lung Disease Models.Biosensors · 2021Review
- Development of Breast Cancer Spheroids to Evaluate Cytotoxic Response to an Anticancer Peptide.Pharmaceutics · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent decades, three-dimensional (3D) cancer cell models have attracted increasing interest in the field of drug screening due to their significant advantages in more accurate simulations of heterogeneous tumor behavior in vivo compared to two-dimensional models. Furthermore, drug sensitivity testing based on 3D cancer cell models can provide more reliable in vivo efficacy prediction. The gold standard fluorescence staining is hard to achieve real-time and label-free viability monitoring in 3D cancer cell models. In this study, a microgroove impedance sensor (MGIS) was specially developed for the dynamic and noninvasive monitoring of 3D cell viability. 3D cancer cells were trapped in microgrooves with gold electrodes on opposite walls for in situ impedance measurement. The change in the number of live cells caused inversely proportional changes to the impedance magnitude of the entire cell/Matrigel construct and reflected the proliferation and apoptosis of the 3D cells. It was confirmed that the 3D cell viability detected by the MGIS was highly consistent with the standard live/dead staining by confocal microscope characterization. Furthermore, the accuracy of the MGIS was validated quantitatively using a 3D lung cancer model and sophisticated drug sensitivity testing. In addition, the parameters of the MGIS in the measurement experiments were optimized in detail using simulations and experimental validation. The results demonstrated that the MGIS coupled with 3D cell culture would be a promising platform to improve the efficiency and accuracy of cell-based anticancer drug screening in vitro.
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