ArticleMicrosystems & nanoengineering2020
AFM-compatible microfluidic platform for affinity-based capture and nanomechanical characterization of circulating tumor cells.
Article in Microsystems & nanoengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Recent advances in integrated biophysical and biochemical microfluidic methods for circulating tumor cells isolation and analysis.Fundamental research · 2026Review
- Circulating tumour cells as a window into lethality in prostate cancer.Nature reviews. Urology · 2026Review
- ASM-chip: an antibody-modified size-screening microfluidic chip for high-efficiency circulating glioma cell isolation and clinical application.Microsystems & nanoengineering · 2026Article
- Nanomechanical Phenotyping of Circulating Tumor Cells with Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Nanomechanical Phenotyping of Circulating Tumor Cells with Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Circulating Tumor Cells for the Monitoring of Lung Cancer Therapies.International journal of molecular sciences · 2025Review
- Microfluidic mixing probe: generating multiple concentration-varying flow dipoles.Scientific reports · 2025Article
- Article
- Computational analysis of cancer cell adhesion in curved vessels affected by wall shear stress for prediction of metastatic spreading.Frontiers in bioengineering and biotechnology · 2024Article
- Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus.Nanomaterials (Basel, Switzerland) · 2023Article
- AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions.ACS measurement science au · 2023Review
- Review
- Lateral force separation of biopolymers using an atomic force microscope.Biomicrofluidics · 2023Article
- Investigation of Soft Matter Nanomechanics by Atomic Force Microscopy and Optical Tweezers: A Comprehensive Review.Nanomaterials (Basel, Switzerland) · 2023Review
- Spike- and nucleocapsid-based gold colloid assay toward the development of an adhesive bandage for rapid SARS-CoV-2 immune response detection and screening.Microsystems & nanoengineering · 2023Article
- Affinity-Based Microfluidics Combined with Atomic Force Microscopy for Isolation and Nanomechanical Characterization of Circulating Tumor Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Next-Generation Microfluidics for Biomedical Research and Healthcare Applications.Biomedical engineering and computational biology · 2023Review
- Article
- Quantifying Deformation and Migration Properties of U87 Glioma Cells Using Dielectrophoretic Forces.Biosensors · 2022Article
- 3D Generation of Multipurpose Atomic Force Microscopy Tips.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circulating tumor cells (CTCs) carried by the patient's bloodstream are known to lead to the metastatic spread of cancer. It is becoming increasingly clear that an understanding of the nanomechanical characteristics of CTCs, such as elasticity and adhesiveness, represents advancements in tracking and monitoring cancer progression and metastasis. In the present work, we describe a combined microfluidic-atomic force microscopy (AFM) platform that uses antibody-antigen capture to routinely isolate and nanomechanically characterize CTCs present in blood samples from prostate cancer patients. We introduce the reversible assembly of a microfluidic device and apply refined and robust chemistry to covalently bond antibodies onto its glass substrate with high density and the desired orientation. As a result, we show that the device can efficiently capture CTCs from patients with localized and metastatic prostate cancer through anti-EpCAM, anti-PSA, and anti-PSMA antibodies, and it is suitable for AFM measurements of captured intact CTCs. When nanomechanically characterized, CTCs originating from metastatic cancer demonstrate decreased elasticity and increased deformability compared to those originating from localized cancer. While the average adhesion of CTCs to the AFM tip surface remained the same in both the groups, there were fewer multiple adhesion events in metastatic CTCs than there were in their counterparts. The developed platform is simple, robust, and reliable and can be useful in the diagnosis and prognosis of prostate cancer as well as other forms of cancer.
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