ArticleMicrosystems & nanoengineering2020
E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum.
Article in Microsystems & nanoengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- A Sensitive DNA Bowtie Sensor for Calibration-Free Sex Hormone Detection Using Analyte-DNA Conjugates and Multiple Redox Labels.ACS measurement science au · 2026Article
- Aptamer-Based Scanning Electrochemical Microscopy for Specific and Localized Detection of Analyte Concentration.ACS electrochemistry · 2026Article
- Next-generation clinically relevant antibody detection: Unlocking electrochemical biosensors for critical disease management.Acta pharmaceutica Sinica. B · 2025Review
- On the Design, Fabrication, and Operation of Electrochemical Aptamer-Based Sensors.ACS sensors · 2025Review
- Cyclooctyne-Functionalized N-Heterocyclic Carbenes for Click Coupling of Sensing Oligonucleotides to Gold Surfaces.ACS sensors · 2025Article
- Multidomain Molecular Sensor Devices, Systems, and Algorithms for Improved Physiological Monitoring.Micromachines · 2025Review
- FRET-based reporter assesses lysosomal DNA-degradation ability in live cells.Sensors and actuators reports · 2025Article
- An electrochemical proximity assay (ECPA) for antibody detection incorporating flexible spacers for improved performance.Analytical and bioanalytical chemistry · 2024Article
- Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing.Advances in biochemical engineering/biotechnology · 2024Review
- Synthetic Antigen-Conjugated DNA Systems for Antibody Detection and Characterization.ACS sensors · 2023Review
- Biomolecular sensors for advanced physiological monitoring.Nature reviews bioengineering · 2023Review
- Conformational-switch biosensors as novel tools to support continuous, real-time molecular monitoring in lab-on-a-chip devices.Lab on a chip · 2023Review
- Determining interchromophore effects for energy transport in molecular networks using machine-learning algorithms.Physical chemistry chemical physics : PCCP · 2023Article
- Human Cyclophilin B Nuclease Activity Revealed via Nucleic Acid-Based Electrochemical Sensors.Angewandte Chemie (International ed. in English) · 2022Article
- Programmable Cell-Free Transcriptional Switches for Antibody Detection.Journal of the American Chemical Society · 2022Article
- Electrochemical diagnostics of infectious viral diseases: Trends and challenges.Biosensors & bioelectronics · 2021Review
- Reagentless biomolecular analysis using a molecular pendulum.Nature chemistry · 2021Article
- An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays.Nano-micro letters · 2021Review
- Beyond Sensitive and Selective Electrochemical Biosensors: Towards Continuous, Real-Time, Antibiofouling and Calibration-Free Devices.Sensors (Basel, Switzerland) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The multiplexed, point-of-care measurement of specific antibodies could improve the speed with which diseases are diagnosed and their treatment initiated. To this end, we are developing E-DNA scaffold sensors, which consist of a rigid, nucleic acid "scaffold" attached on one end to an electrode and presenting both a redox reporter and an epitope on the other. In the absence of antibody, the reporter efficiently transfers electrons when interrogated electrochemically. Binding-induced steric hindrance limits movement, reducing electron transfer in a manner that is both easily measured and quantitatively related to target concentration. Previously we have used monoclonal antibodies to explore the analytical performance of E-DNA sensors, showing that they support the rapid, single-step, quantitative detection of multiple antibodies in small volume samples. Here, in contrast, we employ authentic human samples to better explore the platform's clinical potential. Specifically, we developed E-DNA sensors targeting three HIV-specific antibodies and then compared the analytical and clinical performance of these against those of gold standard serological techniques. Doing so we find that, although the multistep amplification of an ELISA leads to a lower detection limits, the clinical sensitivity of ELISAs, E-DNA sensors and lateral-flow dipsticks are indistinguishable across our test set. It thus appears that, by merging the quantitation and multiplexing of ELISAs with the convenience and speed of dipsticks, E-DNA scaffold sensors could significantly improve on current serological practice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.