ArticleMicrosystems & nanoengineering2020
On-chip multiplexed single-cell patterning and controllable intracellular delivery.
Article in Microsystems & nanoengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- A critical review of microfluidic electroporation for therapeutic cell engineering.Cell reports. Physical science · 2026Article
- Microfluidic-Based Electrical Operation and Measurement Methods in Single-Cell Analysis.Sensors (Basel, Switzerland) · 2024Review
- Integrated Microfluidics for Single-Cell Separation and On-Chip Analysis: Novel Applications and Recent Advances.Small science · 2024Article
- Nanoporous electroporation needle for localized intracellular delivery in deep tissues.Bioengineering & translational medicine · 2023Article
- Current research status of tumor cell biomarker detection.Microsystems & nanoengineering · 2023Review
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- In Situ Electroporation on PERFECT Filter for High-Efficiency and High-Viability Tumor Cell Labeling.Micromachines · 2022Article
- Cellular nanointerface of vertical nanostructure arrays and its applications.Nanoscale advances · 2022Review
- Modeling the gene delivery process of the needle array-based tissue nanotransfection.Nano research · 2022Article
- An equivalent circuit model for localized electroporation on porous substrates.Biosensors & bioelectronics · 2022Article
- A microexplosive shockwave-based drug delivery microsystem for treating hard-to-reach areas in the human body.Microsystems & nanoengineering · 2022Article
- Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework.ACS omega · 2021Article
- Microfluidic and Nanofluidic Intracellular Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Micro/nanodevices for assessment and treatment in stomatology and ophthalmology.Microsystems & nanoengineering · 2021Review
- High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Is microfluidics the "assembly line" for CRISPR-Cas9 gene-editing?Biomicrofluidics · 2020Article
- Single-cell individualized electroporation with real-time impedance monitoring using a microelectrode array chip.Microsystems & nanoengineering · 2020Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional electroporation approaches show limitations in the delivery of macromolecules in vitro and in vivo. These limitations include low efficiency, noticeable cell damage and nonuniform delivery of cells. Here, we present a simple 3D electroporation platform that enables massively parallel single-cell manipulation and the intracellular delivery of macromolecules and small molecules. A pyramid pit micropore array chip was fabricated based on a silicon wet-etching method. A controllable vacuum system was adopted to trap a single cell on each micropore. Using this chip, safe single-cell electroporation was performed at low voltage. Cargoes of various sizes ranging from oligonucleotides (molecular beacons, 22 bp) to plasmid DNA (CRISPR-Cas9 expression vectors, >9 kb) were delivered into targeted cells with a significantly higher transfection efficiency than that of multiple benchmark methods (e.g., commercial electroporation devices and Lipofectamine). The delivered dose of the chemotherapeutic drug could be controlled by adjusting the applied voltage. By using CRISPR-Cas9 transfection with this system, the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.